Evidence map›Paper›PMID 27102682›Full record

ArticleNature chemistry2016

Self-assembly of size-controlled liposomes on DNA nanotemplates.

Yang Yang, Jing Wang, Hideki Shigematsu, Weiming Xu, William M Shih, James E Rothman, Chenxiang Lin

Abstract read
In one paragraph

Article in Nature chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.

0numbers the graph read from it
0cells of the map it votes in
76citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

76 citing papers in PubMed.

  1. Controlling membrane tension by dynamic DNA nanorings.bioRxiv : the preprint server for biology · 2026
    Article
  2. Review
  3. Review
  4. Article
  5. DNA-Lipid Nanodiscs with a Polyethylene Glycol Interface.Journal of the American Chemical Society · 2026
    Article
  6. Review
  7. Programmable Liposome Organization via DNA Origami Templates.Journal of the American Chemical Society · 2025
    Article
  8. Review
  9. Article
  10. Review
  11. DNA-Assisted Assays for Studying Lipid Transfer Between Membranes.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  12. Article
  13. Article
  14. Review
  15. DNA-based nanostructures for RNA delivery.Medical review (2021) · 2024
    Review
  16. Review
  17. Article
  18. Article
  19. Article
  20. Review

16 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yang YangDepartment of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.ORCID 0000-0002-2630-6062
Jing WangDepartment of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Hideki ShigematsuDepartment of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06250, USA.
Weiming XuDepartment of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
William M ShihWyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts 02138, USA.
James E RothmanDepartment of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Chenxiang LinDepartment of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

Funding

Regulation of Membrane Fusion in ExocytosisR01DK027044 · NIDDK · YALE UNIVERSITY · PI KRISHNAKUMAR, SHYAM S · 1986 to 2021
$8.2M
NMR Structure Determination of Membrane Proteins Enabled by DNA NanotubesDP2OD004641 · OD · DANA-FARBER CANCER INST · PI SHIH, WILLIAM M · 2008 to 2008
$2.6M
Cell-free membrane remodeling guided by DNA nano-templatesDP2GM114830 · NIGMS · YALE UNIVERSITY · PI LIN, CHENXIANG · 2014 to 2014
$2.5M
Generating nuclear pore complex mimics with DNA origamiR21GM109466 · NIGMS · YALE UNIVERSITY · PI LIN, CHENXIANG, LUSK, CHARLES PATRICK · 2014 to 2015
$458k
NIDDK NIH HHS R01 DK027044NIGMS NIH HHS DP2 GM114830NIGMS NIH HHS R21 GM109466NIH HHS DP2 OD004641
6 · The paper itself

Abstract

Artificial lipid-bilayer membranes are valuable tools for the study of membrane structure and dynamics. For applications such as the study of vesicular transport and drug delivery, there is a pressing need for artificial vesicles with controlled size. However, controlling vesicle size and shape with nanometre precision is challenging, and approaches to achieve this can be heavily affected by lipid composition. Here, we present a bio-inspired templating method to generate highly monodispersed sub-100-nm unilamellar vesicles, where liposome self-assembly was nucleated and confined inside rigid DNA nanotemplates. Using this method, we produce homogeneous liposomes with four distinct predefined sizes. We also show that the method can be used with a variety of lipid compositions and probe the mechanism of templated liposome formation by capturing key intermediates during membrane self-assembly. The DNA nanotemplating strategy represents a conceptually novel way to guide lipid bilayer formation and could be generalized to engineer complex membrane/protein structures with nanoscale precision.

Indexed as

DNAFatty Acids, MonounsaturatedLipid BilayersNanostructuresParticle SizePhosphatidylcholinesPhosphatidylserinesPolyethylene GlycolsQuaternary Ammonium CompoundsUnilamellar Liposomes1,2-dioleoyloxy-3-(trimethylammonium)propane1,2-dioleoylphosphatidylserine1,2-oleoylphosphatidylcholineDNAFatty Acids, MonounsaturatedLipid BilayersPhosphatidylcholinesPhosphatidylserinesPolyethylene GlycolsQuaternary Ammonium CompoundsUnilamellar Liposomes

Identifiers

PMID27102682
PMCPMC5021307

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.