ArticleParasitology research2016
Isolation and genotyping of Acanthamoeba strains (T4, T9, and T11) from amoebic keratitis patients in Iran.
Article in Parasitology research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 56 citations in OpenAlex.
- Global prevalence of potentially pathogenic free-living amoebae in sewage and sewage-related environments-systematic review with meta-analysis.Parasitology research · 2024Pooled it
- Genotype distribution of Acanthamoeba in keratitis: a systematic review.Parasitology research · 2021Pooled it
- Effect of aqueous extract of Eucalyptus Microtheca on Acanthamoeba genotype T5, an in vitro study.BMC complementary medicine and therapies · 2025Article
- Prevalence of free-living amoebae in five rivers associated with high human activity in Tehran province, Iran.Journal of water and health · 2025Article
- Identification and Characterization of Acanthamoeba from Contact Lens Care Solutions and Hospital Environments in the Ophthalmology Wards and Operating Rooms in Southeastern Iran.Acta parasitologica · 2025Article
- Assessment ofJournal of water and health · 2024Article
- Morphological Characterization and Genotyping ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2024Article
- Assessment of pathogenic potential of Acanthamoeba isolates by in vitro and in vivo tests.Parasitology research · 2023Article
- In Vitro Cytopathogenic Activities ofPathogens (Basel, Switzerland) · 2022Article
- Article
- On the diversity and clinical importance of Acanthamoeba spp. from Group 1.Parasitology research · 2021Article
- Detection and Identification ofJournal of clinical microbiology · 2021Article
- Genotyping and phylogenic study ofParasite epidemiology and control · 2020Article
- Free-living amoebae and their relationship to air quality in hospital environments: characterization ofParasitology · 2020Article
- Isolation, identification, and phylogenetic analysis of potentially pathogenic free-living amoebae isolated from nasal and oral mucosa of HIV/AIDS patients in Iran.Parasitology research · 2019Article
- Effectiveness of sampling methods employed for Acanthamoeba keratitis diagnosis by culture.International ophthalmology · 2019Observational
- Genotypic diversity of Acanthamoeba strains isolated from Chilean patients with Acanthamoeba keratitis.Parasites & vectors · 2019Article
- Isolation and identification of Acanthamoeba from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters.BMC microbiology · 2018Article
- Genotyping ofThe Indian journal of medical research · 2018Article
- Occurrence of Free-living Amoebae in Nasal Swaps of Patients of Intensive Care Unit (ICU) and Critical Care Unit (CCU) and Their Surrounding Environments.Iranian journal of public health · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The continuous increase in Acanthamoeba keratitis, a severe corneal infection, worldwide is mainly due to the increase in the number of soft contact lens users. In the present study, which involves a 5-year study, a total of 138 corneal scraps and contact lenses together with their paraphernalias were obtained from suspected amoebic keratitis patients. All samples were cultured using culture-enrichment method. Pathogenic assay, using thermotolerance and osmotolerance tests were also performed on the positive strains. Sequencing of the isolated strains was done by targeting the DF3 region of 18s rRNA gene. The results revealed that 18 (13 %) of patients were infected with Acanthamoeba spp. As expected, T4 genotype was the most common genotype among the clinical samples; however, in three cases, Acanthamoeba belonging to T11 and T9 were detected. Interestingly, T9 genotype, commonly classified as non-pathogenic amoebae, was identified as a causal agent of a patient with amoebic keratitis. From the pathogenic assay, four strains belonging to T4 genotypes were highly pathogenic. This is the first report of Acanthamoeba T9 genotypes isolated in Iran and the first report of T9 type occurring in amoebic keratitis patients worldwide. Due to the increasing trend of amoebic keratitis (AK) and the identification of new genotypes, such as T9 as the causative agent of AK, more researches in this field are necessary in the region and the world at large.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.