ArticleJournal of neuroscience methods2016
Intact skull chronic windows for mesoscopic wide-field imaging in awake mice.
Article in Journal of neuroscience methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
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Who cites it
115 citing papers in PubMed, 222 citations in OpenAlex.
- Real-time closed-loop feedback system for mouse mesoscale cortical signal and movement control.eLife · 2026Article
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- High-fidelity intravital imaging of biological dynamics with latent-space-enhanced digital adaptive optics.Nature biotechnology · 2026Article
- Choosing a skull clearing technique for chronic mesoscopic optical imaging in awake mice.Neurophotonics · 2026Article
- Hemodynamic and Electrophysiological Progression of the Rose Bengal Photothrombotic Stroke Model in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Neural barcoding representing cortical spatiotemporal dynamics based on continuous-time Markov chains.Cell reports methods · 2026Article
- Pupillometry and brain-wide c-Fos mapping uncover multimodal mirror emotional contagion related networks of mice.iScience · 2026Article
- Smooth Muscle Dysfunction Drives Cerebrovascular Reserve Failure and End-Organ Brain Injury.bioRxiv : the preprint server for biology · 2026Article
- The protocol for mesoscopic wide-field optical imaging in mice: from zero to hero.Biology methods & protocols · 2026Review
- Sim-to-real domain adaptation based completion level recognition for autonomous micro-drilling in biomedical application.Scientific reports · 2025Article
- Characterization of iGABASnFR2 forNeurophotonics · 2025Article
- Computer vision-guided rapid and precise automated cranial microsurgeries in mice.Science advances · 2025Article
- Contralesional hippocampal spreading depolarization promotes functional recovery after stroke.Nature communications · 2025Article
- Multiplexed subspaces route neural activity across brain-wide networks.Nature communications · 2025Article
- Microvascular structure variability explains variance in fMRI functional connectivity.Brain structure & function · 2025Article
- Functional connectivity changes in mouse models of maple syrup urine disease.Cerebral cortex (New York, N.Y. : 1991) · 2025Article
- Wearable optical coherence tomography angiography probe with extended depth of field.Journal of biomedical optics · 2025Article
55 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundCraniotomy-based window implants are commonly used for microscopic imaging, in head-fixed rodents, however their field of view is typically small and incompatible with mesoscopic functional mapping of cortex. NEW
methodWe describe a reproducible and simple procedure for chronic through-bone wide-field imaging in awake head-fixed mice providing stable optical access for chronic imaging over large areas of the cortex for months.
resultsThe preparation is produced by applying clear-drying dental cement to the intact mouse skull, followed by a glass coverslip to create a partially transparent imaging surface. Surgery time takes about 30min. A single set-screw provides a stable means of attachment (in relation to the measured lateral and axial resolution) for mesoscale assessment without obscuring the cortical field of view. COMPARISON WITH EXISTING
methodsWe demonstrate the utility of this method by showing seed-pixel functional connectivity maps generated from spontaneous cortical activity of GCAMP6 signals in both awake and anesthetized mice in longitudinal studies of up to 2 months in duration.
conclusionsWe propose that the intact skull preparation described here may be used for most longitudinal studies that do not require micron scale resolution and where cortical neural or vascular signals are recorded with intrinsic sensors or in transgenic mice expressing genetically encoded sensors of activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.