ArticlePloS one2016
Isolation and Functional Validation of Salinity and Osmotic Stress Inducible Promoter from the Maize Type-II H+-Pyrophosphatase Gene by Deletion Analysis in Transgenic Tobacco Plants.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 46 citations in OpenAlex.
- Article
- From Structure to Function of Promoters and 5'UTRs in Maize.International journal of molecular sciences · 2026Review
- Genetic dissection of crown rust resistance in oat and the identification of key adult plant resistance genes.The plant genome · 2025Article
- Isolation and Characterization ofPlants (Basel, Switzerland) · 2023Article
- Isolation and characterization of drought and ABA responsive promoter of a transcription factor encoding gene from rice.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Isolation and characterization of maizePhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Identification of transcription factors interacting with a 1274 bp promoter of MaPIP1;1 which confers high-level gene expression and drought stress Inducibility in transgenic Arabidopsis thaliana.BMC plant biology · 2020Article
- MTA, an RNA mInternational journal of molecular sciences · 2020Article
- An update and perspectives on the use of promoters in plant genetic engineering.Journal of biosciences · 2020Review
- Isolation and functional characterization of three abiotic stress-inducible (Apx, Dhn and Hsc70) promoters from pearl millet (Pennisetum glaucum L.).Molecular biology reports · 2019Article
- Identification of Flower-Specific Promoters through Comparative Transcriptome Analysis inInternational journal of molecular sciences · 2019Article
- Genome-wide identification of CAMTA gene family members in Phaseolus vulgaris L. and their expression profiling during salt stress.Molecular biology reports · 2019Article
- Isolation and functional validation of the CmLOX08 promoter associated with signalling molecule and abiotic stress responses in oriental melon, Cucumis melo var. makuwa Makino.BMC plant biology · 2019Article
- Isolation and identification of a vegetative organ-specific promoter from maize.Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Article
- Characterization of a strong and constitutive promoter from the Arabidopsis serine carboxypeptidase-like gene AtSCPL30 as a potential tool for crop transgenic breeding.BMC biotechnology · 2018Article
- Stress Inducible Overexpression ofFrontiers in chemistry · 2018Article
- Genome-wide analysis of3 Biotech · 2017Article
- Functional Characterization of Tea (Frontiers in plant science · 2017Article
- Functional Characterization ofFrontiers in plant science · 2017Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salinity and drought severely affect both plant growth and productivity, making the isolation and characterization of salinity- or drought-inducible promoters suitable for genetic improvement of crop resistance highly desirable. In this study, a 1468-bp sequence upstream of the translation initiation codon ATG of the promoter for ZmGAPP (maize Type-II H+-pyrophosphatase gene) was cloned. Nine 5´ deletion fragments (D1-D9) of different lengths of the ZmGAPP promoter were fused with the GUS reporter and translocated into tobacco. The deletion analysis showed that fragments D1-D8 responded well to NaCl and PEG stresses, whereas fragment D9 and CaMV 35S did not. The D8 segment (219 bp; -219 to -1 bp) exhibited the highest promoter activity of all tissues, with the exception of petals among the D1-D9 transgenic tobacco, which corresponds to about 10% and 25% of CaMV 35S under normal and NaCl or PEG stress conditions, respectively. As such, the D8 segment may confer strong gene expression in a salinity and osmotic stress inducible manner. A 71-bp segment (-219 to -148 bp) was considered as the key region regulating ZmGAPP response to NaCl or PEG stress, as transient transformation assays demonstrated that the 71-bp sequence was sufficient for the salinity or osmotic stress response. These results enhance our understanding of the molecular mechanisms regulating ZmGAPP expression, and that the D8 promoter would be an ideal candidate for moderating expression of drought and salinity response genes in transgenic plants.
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