ArticlePloS one2016
Intracellular Delivery of Proteins via Fusion Peptides in Intact Plants.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 72 citations in OpenAlex.
- C-Terminal Nucleobase Modification Amplifies Peptide-Mediated Liposome Fusion.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Nanoparticle-Mediated Nucleic Acid Delivery Systems in Plant Biotechnology: Recent Advances and Emerging Challenges.Plants (Basel, Switzerland) · 2025Review
- Cell-Penetrating Peptide-Mediated Delivery of Gene-Silencing Nucleic Acids to the Invasive Common ReedPlants (Basel, Switzerland) · 2025Article
- Cell-penetrating peptides for sustainable agriculture.Trends in plant science · 2024Review
- Towards realizing nano-enabled precision delivery in plants.Nature nanotechnology · 2024Review
- Ampholytic Peptides Consisting of an Alternating Lysine/Glutamic Acid Sequence for the Simultaneous Formation of Polyion Complex Vesicles.ACS polymers Au · 2024Article
- Nanoplatforms for the Delivery of Nucleic Acids into Plant Cells.International journal of molecular sciences · 2023Review
- Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration.Frontiers in genome editing · 2023Review
- Genome editing by introduction of Cas9/sgRNA into plant cells using temperature-controlled atmospheric pressure plasma.PloS one · 2023Article
- Nano-enabled agriculture: How do nanoparticles cross barriers in plants?Plant communications · 2022Review
- Nanomaterials coupled with microRNAs for alleviating plant stress: a new opening towards sustainable agriculture.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Review
- Non-transgenic Gene ModulationACS nano · 2022Article
- Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Transgene-free genome editing and RNAi ectopic application in fruit trees: Potential and limitations.Frontiers in plant science · 2022Review
- Applications of CPPs in Genome Editing of Plants.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Recent Developments and Strategies for the Application ofFrontiers in plant science · 2022Review
- Functional peptide-mediated plastid transformation in tobacco, rice, and kenaf.Frontiers in plant science · 2022Article
- Nanoparticles for protein delivery in planta.Current opinion in plant biology · 2021Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In current plant biotechnology, the introduction of exogenous DNA encoding desired traits is the most common approach used to modify plants. However, general plant transformation methods can cause random integration of exogenous DNA into the plant genome. To avoid these events, alternative methods, such as a direct protein delivery system, are needed to modify the plant. Although there have been reports of the delivery of proteins into cultured plant cells, there are currently no methods for the direct delivery of proteins into intact plants, owing to their hierarchical structures. Here, we demonstrate the efficient fusion-peptide-based delivery of proteins into intact Arabidopsis thaliana. Bovine serum albumin (BSA, 66 kDa) was selected as a model protein to optimize conditions for delivery into the cytosol. The general applicability of our method to large protein cargo was also demonstrated by the delivery of alcohol dehydrogenase (ADH, 150 kDa) into the cytosol. The compatibility of the fusion peptide system with the delivery of proteins to specific cellular organelles was also demonstrated using the fluorescent protein Citrine (27 kDa) conjugated to either a nuclear localization signal (NLS) or a peroxisomal targeting signal (PTS). In conclusion, our designed fusion peptide system can deliver proteins with a wide range of molecular weights (27 to 150 kDa) into the cells of intact A. thaliana without interfering with the organelle-targeting peptide conjugated to the protein. We expect that this efficient protein delivery system will be a powerful tool in plant biotechnology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.