ArticleJournal of experimental botany2016
A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Advances in Plant Antiviral RNAi: From Host DCLs/RDRs to Diversified Viral Counteracting Strategies.Viruses · 2026Review
- Antiviral RNA interference in plants: Increasing complexity and integration with other biological processes.Plant communications · 2025Review
- PhWRKY30 activates salicylic acid biosynthesis to positively regulate antiviral defense response in petunia.Horticulture research · 2025Article
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- The Role of Plant Transcription Factors in the Fight against Plant Viruses.International journal of molecular sciences · 2023Review
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- Transcriptome Profiling Reveals a Petunia Transcription Factor,Frontiers in plant science · 2022Article
- Genome-Wide Identification and Expression Profile Analysis of the NF-Y Transcription Factor Gene Family inPlants (Basel, Switzerland) · 2020Article
- Comparative transcriptome profiling uncovers a Lilium regale NAC transcription factor, LrNAC35, contributing to defence response against cucumber mosaic virus and tobacco mosaic virus.Molecular plant pathology · 2019Article
- Effects of Abscisic Acid and Salicylic Acid on Gene Expression in the Antiviral RNA Silencing Pathway in Arabidopsis.International journal of molecular sciences · 2019Article
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- RrGT2, A Key Gene Associated with Anthocyanin Biosynthesis in Rosa rugosa, Was Identified Via Virus-Induced Gene Silencing and Overexpression.International journal of molecular sciences · 2018Article
- Transcriptomic and Functional Analyses Reveal ThatFrontiers in plant science · 2018Article
- SAGA complex mediates the transcriptional up-regulation of antiviral RNA silencing.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- PhOBF1, a petunia ocs element binding factor, plays an important role in antiviral RNA silencing.Journal of experimental botany · 2017Article
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Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Virus-induced RNA silencing is involved in plant antiviral defense and requires key enzyme components, including RNA-dependent RNA polymerases (RDRs), Dicer-like RNase III enzymes (DCLs), and Argonaute proteins (AGOs). However, the transcriptional regulation of these critical components is largely unknown. In petunia (Petunia hybrida), an ethylene-responsive element binding factor, PhERF2, is induced by Tobacco rattle virus (TRV) infection. Inclusion of a PhERF2 fragment in a TRV silencing construct containing reporter fragments of phytoene desaturase (PDS) or chalcone synthase (CHS) substantially impaired silencing efficiency of both the PDS and CHS reporters. Silencing was also impaired in PhERF2- RNAi lines, where TRV-PhPDS infection did not show the expected silencing phenotype (photobleaching). In contrast, photobleaching in response to infiltration with the TRV-PhPDS construct was enhanced in plants overexpressing PhERF2 Transcript abundance of the RNA silencing-related genes RDR2, RDR6, DCL2, and AGO2 was lower in PhERF2-silenced plants but higher in PhERF2-overexpressing plants. Moreover, PhERF2-silenced lines showed higher susceptibility to Cucumber mosaic virus (CMV) than wild-type (WT) plants, while plants overexpressing PhERF2 exhibited increased resistance. Interestingly, growth and development of PhERF2-RNAi lines were substantially slower, whereas the overexpressing lines were more vigorous than the controls. Taken together, our results indicate that PhERF2 functions as a positive regulator in antiviral RNA silencing.
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