ArticleGenetics2016
Efficient Genome-Wide Sequencing and Low-Coverage Pedigree Analysis from Noninvasively Collected Samples.
Article in Genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 97 citations in OpenAlex.
- Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids.Journal of fish biology · 2026Review
- Maximizing the Quality of Non-Invasive Samples for Conservation Genetics Using Targeted Next-Generation Sequencing: A Comparison of Fecal DNA Preservation Methodologies.Ecology and evolution · 2026Article
- DNA purity over yield: optimizing extraction and PCR in degraded Asian elephant (Elephas maximus) fecal samples.Genes & genomics · 2026Article
- Genetic legacy and recent cross of two ancient lineages underlie the rebound of the world's rarest primate.Science advances · 2026Article
- Age Is Just a Number: No Impacts of Scat Ageing on Single Nucleotide Polymorphism Genotyping Using a Target Capture Approach.Ecology and evolution · 2025Article
- Comparison of Fecal DNA Extraction Kits for the Giant Panda (Ecology and evolution · 2025Article
- Estimating realized relatedness in free-ranging macaques by inferring identity-by-descent segments.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- FecalSeq enrichment with RAD Sequencing from non-invasive environmental samples holds promise for genetic monitoring of an imperiled lagomorph.Scientific reports · 2024Article
- Review
- Reflections on connections.Primates; journal of primatology · 2023Article
- Review
- Selection against admixture and gene regulatory divergence in a long-term primate field study.Science (New York, N.Y.) · 2022Article
- Genotyping-in-thousands by sequencing (GT-seq) of noninvasive faecal and degraded samples: A new panel to enable ongoing monitoring of Canadian polar bear populations.Molecular ecology resources · 2022Article
- Potential drivers of samango monkey (Cercopithecus albogularis) population subdivision in a highly fragmented mountain landscape in northern South Africa.Primates; journal of primatology · 2022Article
- Combining methods for non-invasive fecal DNA enables whole genome and metagenomic analyses in wildlife biology.Frontiers in genetics · 2022Article
- Epigenetic models developed for plains zebras predict age in domestic horses and endangered equids.Communications biology · 2021Article
- Pedigree reconstruction and distant pairwise relatedness estimation from genome sequence data: A demonstration in a population of rhesus macaques (Macaca mulatta).Molecular ecology resources · 2021Article
- Genome-wide ancestry and introgression in a Zambian baboon hybrid zone.Molecular ecology · 2021Article
- The genomics of ecological flexibility, large brains, and long lives in capuchin monkeys revealed with fecalFACS.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Urine as a high-quality source of host genomic DNA from wild populations.Molecular ecology resources · 2021Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 5 countries.
Funding
Abstract
Research on the genetics of natural populations was revolutionized in the 1990s by methods for genotyping noninvasively collected samples. However, these methods have remained largely unchanged for the past 20 years and lag far behind the genomics era. To close this gap, here we report an optimized laboratory protocol for genome-wide capture of endogenous DNA from noninvasively collected samples, coupled with a novel computational approach to reconstruct pedigree links from the resulting low-coverage data. We validated both methods using fecal samples from 62 wild baboons, including 48 from an independently constructed extended pedigree. We enriched fecal-derived DNA samples up to 40-fold for endogenous baboon DNA and reconstructed near-perfect pedigree relationships even with extremely low-coverage sequencing. We anticipate that these methods will be broadly applicable to the many research systems for which only noninvasive samples are available. The lab protocol and software ("WHODAD") are freely available at www.tung-lab.org/protocols-and-software.html and www.xzlab.org/software.html, respectively.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.