ArticleeLife2016
Synthetic protein interactions reveal a functional map of the cell.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Proteome-wide forced interactions reveal a functional map of cell-cycle phospho-regulation inNucleus (Austin, Tex.) · 2024Article
- Yeast goes viral: probing SARS-CoV-2 biology usingMicrobial cell (Graz, Austria) · 2022Article
- ScreenGarden: a shinyR application for fast and easy analysis of plate-based high-throughput screens.BMC bioinformatics · 2022Article
- Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking.Microbial cell (Graz, Austria) · 2021Article
- Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast.Molecular biology of the cell · 2021Article
- Application of Fluorescent Proteins for Functional Dissection of theInternational journal of molecular sciences · 2021Review
- A platform for post-translational spatiotemporal control of cellular proteins.Synthetic biology (Oxford, England) · 2021Article
- Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA.PLoS genetics · 2020Article
- Nanobody-Based Probes for Subcellular Protein Identification and Visualization.Frontiers in cellular neuroscience · 2020Review
- Synthetic Physical Interactions with the Yeast Centrosome.G3 (Bethesda, Md.) · 2019Article
- A nanobody-based toolset to investigate the role of protein localization and dispersal ineLife · 2017Article
- CRISPR-UnLOCK: Multipurpose Cas9-Based Strategies for Conversion of Yeast Libraries and Strains.Frontiers in microbiology · 2017Article
- Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy.Frontiers in immunology · 2017Review
- Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation Regions.G3 (Bethesda, Md.) · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
To understand the function of eukaryotic cells, it is critical to understand the role of protein-protein interactions and protein localization. Currently, we do not know the importance of global protein localization nor do we understand to what extent the cell is permissive for new protein associations - a key requirement for the evolution of new protein functions. To answer this question, we fused every protein in the yeast Saccharomyces cerevisiae with a partner from each of the major cellular compartments and quantitatively assessed the effects upon growth. This analysis reveals that cells have a remarkable and unanticipated tolerance for forced protein associations, even if these associations lead to a proportion of the protein moving compartments within the cell. Furthermore, the interactions that do perturb growth provide a functional map of spatial protein regulation, identifying key regulatory complexes for the normal homeostasis of eukaryotic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.