SynthesisNeurological research2016
Histological methods for ex vivo axon tracing: A systematic review.
Synthesis in Neurological research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed.
- Long-range axon branching: contributions to brain network plasticity and repair.Nature reviews. Neuroscience · 2026Review
- Over 30 Years of DiI Use for Human Neuroanatomical Tract Tracing: A Scoping Review.Biomolecules · 2024Article
- Enteric Nervous System: Identification of a Novel Neuronal Sensory Network in the Duodenal Epithelium.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- versaFlow: a versatile pipeline for resolution adapted diffusion MRI processing and its application to studying the variability of the PRIME-DE database.Frontiers in neuroinformatics · 2023Article
- Testing the suitability of neuroanatomical tracing method in human fetuses with long years of postmortem delay.Surgical and radiologic anatomy : SRA · 2022Article
- In vivo MRI evaluation of anterograde manganese transport along the visual pathway following whole eye transplantation.Journal of neuroscience methods · 2022Article
- Developmental Hypothyroidism Influences the Development of the Entorhinal-Dentate Gyrus Pathway of Rat Offspring.Endocrinology and metabolism (Seoul, Korea) · 2022Article
- Kisspeptin-1 regulates forebrain dopaminergic neurons in the zebrafish.Scientific reports · 2020Article
- Brain Wiring and Supragranular-Enriched Genes Linked to Protracted Human Frontal Cortex Development.Cerebral cortex (New York, N.Y. : 1991) · 2020Article
- Closing the gap from transcription to the structural connectome enhances the study of connections in the human brain.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Review
- Pathological application of carbocyanine dye-based multicolour imaging of vasculature and associated structures.Scientific reports · 2020Article
- Neuroanatomical tract-tracing techniques that did go viral.Brain structure & function · 2020Review
- High Angular Resolution Diffusion MRI Reveals Conserved and Deviant Programs in the Paths that Guide Human Cortical Circuitry.Cerebral cortex (New York, N.Y. : 1991) · 2020Article
- Anatomy and Connectivity of the Subthalamic Nucleus in Humans and Non-human Primates.Frontiers in neuroanatomy · 2020Review
- Article
- Towards Differential Connectomics with NeuroVIISAS.Neuroinformatics · 2019Article
- When tractography meets tracer injections: a systematic study of trends and variation sources of diffusion-based connectivity.Brain structure & function · 2018Article
- Optogenetic Tractography for anatomo-functional characterization of cortico-subcortical neural circuits in non-human primates.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
objectivesAxon tracers provide crucial insight into the development, connectivity, and function of neural pathways. A tracer can be characterized as a substance that allows for the visualization of a neuronal pathway. Axon tracers have previously been used exclusively with in vivo studies; however, newer methods of axon tracing can be applied to ex vivo studies. Ex vivo studies involve the examination of cells or tissues retrieved from an organism. These post mortem methods of axon tracing offer several advantages, such as reaching inaccessible tissues and avoiding survival surgeries.
methodsIn order to evaluate the quality of the ex vivo tracing methods, we performed a systematic review of various experimental and comparison studies to discern the optimal method of axon tracing.
resultsThe most prominent methods for ex vivo tracing involve enzymatic techniques or various dyes. It appears that there are a variety of techniques and conditions that tend to give better fluorescent character, clarity, and distance traveled in the neuronal pathway. We found direct comparison studies that looked at variables such as the type of tracer, time required, effect of temperature, and presence of calcium, however, there are other variables that have not been compared directly. DISCUSSION: We conclude there are a variety of promising tracing methods available depending on the experimental goals of the researcher, however, more direct comparison studies are needed to affirm the optimal method.
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