ArticleBMC plant biology2016
Analysis of wheat microspore embryogenesis induction by transcriptome and small RNA sequencing using the highly responsive cultivar "Svilena".
Article in BMC plant biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 51 citations in OpenAlex.
- Integrated transcriptomic and metabolomic analyses reveal phytohormone signaling mechanisms underlying differential regeneration potential in microspore-derived calli from hulled and hulless barley.BMC plant biology · 2026Article
- Transcriptomic analyses reveal regulatory plasticity and metabolic reprogramming underlying genotype-specific microspore embryogenesis in wheat.Plant cell reports · 2026Article
- Targeting histone acetylation to enhance somatic embryogenesis in Quercus suber L.Tree physiology · 2025Article
- Induction of microspore embryogenesis in bread wheat by mannitol pre-treatment is associated with the disruption of endogenous hormone balance and substantial accumulation of auxins.BMC plant biology · 2025Article
- Microinjection of the CRISPR/Cas9 editing system through the germ pore of a wheat microspore induces mutations in the target Ms2 gene.Molecular biology reports · 2024Article
- The Class III Peroxidase geneFrontiers in plant science · 2024Article
- The effect of phytosulfokine alpha on haploid embryogenesis and gene expression ofFrontiers in plant science · 2024Article
- Transcriptomics integrated with metabolomics reveals the effect of cold stress on rice microspores.BMC plant biology · 2023Article
- Phospholipase C is a novel regulator at the early stages of microspore embryogenesis inPlant signaling & behavior · 2022Article
- Comprehensive, integrative genomic analysis of microRNA expression profiles in different tissues of two wheat cultivars with different traits.Functional & integrative genomics · 2022Article
- Microspore embryogenesis induction by mannitol and TSA results in a complex regulation of epigenetic dynamics and gene expression in bread wheat.Frontiers in plant science · 2022Article
- Article
- Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment.Scientific reports · 2021Article
- Reinvestigation of THOUSAND-GRAIN WEIGHT 6 grain weight genes in wheat and rice indicates a role in pollen development rather than regulation of auxin content in grains.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- MicroRNA Omics Analysis ofBiomolecules · 2021Article
- Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives.Frontiers in plant science · 2021Review
- The commitment of barley microspores into embryogenesis correlates with miRNA-directed regulation of members of the SPL, GRF and HD-ZIPIII transcription factor families.Plant direct · 2020Article
- Differential Expression Profiling Reveals Stress-Induced Cell Fate Divergence in Soybean Microspores.Plants (Basel, Switzerland) · 2020Article
- Trichostatin A Affects Developmental Reprogramming of Bread Wheat Microspores towards an Embryogenic Route.Plants (Basel, Switzerland) · 2020Article
- Research Tools for the Functional Genomics of Plant miRNAs During Zygotic and Somatic Embryogenesis.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrospore embryogenesis describes a stress-induced reprogramming of immature male plant gametophytes to develop into embryo-like structures, which can be regenerated into doubled haploid plants after whole genome reduplication. This mechanism is of high interest for both research as well as plant breeding. The objective of this study was to characterize transcriptional changes and regulatory relationships in early stages of cold stress-induced wheat microspore embryogenesis by transcriptome and small RNA sequencing using a highly responsive cultivar.
resultsTranscriptome and small RNA sequencing was performed in a staged time-course to analyze wheat microspore embryogenesis induction. The analyzed stages were freshly harvested, untreated uninucleate microspores and the two following stages from in vitro anther culture: directly after induction by cold-stress treatment and microspores undergoing the first nuclear divisions. A de novo transcriptome assembly resulted in 29,388 contigs distributing to 20,224 putative transcripts of which 9,305 are not covered by public wheat cDNAs. Differentially expressed transcripts and small RNAs were identified for the stage transitions highlighting various processes as well as specific genes to be involved in microspore embryogenesis induction.
conclusionThis study establishes a comprehensive functional genomics resource for wheat microspore embryogenesis induction and initial understanding of molecular mechanisms involved. A large set of putative transcripts presumably specific for microspore embryogenesis induction as well as contributing processes and specific genes were identified. The results allow for a first insight in regulatory roles of small RNAs in the reprogramming of microspores towards an embryogenic cell fate.
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