ArticleNature communications2016
Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Phosphoregulation of the novel hemi-arrestin MAPK scaffold Sms1 prevents untimely mating.Nature communications · 2026Article
- Basal association of a transcription factor favors early gene expression.PLoS genetics · 2025Article
- Multimodal Nanoplasmonic and Fluorescence Imaging for Simultaneous Monitoring of Single-Cell Secretory and Intracellular Dynamics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Density physics-informed neural networks reveal sources of cell heterogeneity in signal transduction.Patterns (New York, N.Y.) · 2024Article
- MaxCal can infer models from coupled stochastic trajectories of gene expression and cell division.Biophysical journal · 2023Article
- Positive feedback induces switch between distributive and processive phosphorylation of Hog1.Nature communications · 2023Article
- When yeast cells change their mind: cell cycle "Start" is reversible under starvation.The EMBO journal · 2023Article
- The modular pYT vector series employed for chromosomal gene integration and expression to produce carbazoles and glycolipids inFEMS microbes · 2023Article
- Quantification of Dark Protein Populations in Fluorescent Proteins by Two-Color Coincidence Detection and Nanophotonic Manipulation.The journal of physical chemistry. B · 2022Article
- Use of red, far-red, and near-infrared light in imaging of yeasts and filamentous fungi.Applied microbiology and biotechnology · 2022Review
- Critical Comparison of MaxCal and Other Stochastic Modeling Approaches in Analysis of Gene Networks.Entropy (Basel, Switzerland) · 2021Article
- Genetically Encoded Fluorescent Biosensor for Rapid Detection of Protein Expression.ACS synthetic biology · 2020Article
- Single-particle imaging of stress-promoters induction reveals the interplay between MAPK signaling, chromatin and transcription factors.Nature communications · 2020Article
- Evidence for rate-dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells.Molecular systems biology · 2020Article
- Cell-in-the-loop pattern formation with optogenetically emulated cell-to-cell signaling.Nature communications · 2020Article
- Quantitative relationships between SMAD dynamics and target gene activation kinetics in single live cells.Scientific reports · 2019Article
- Article
- An Optogenetic Platform for Real-Time, Single-Cell Interrogation of Stochastic Transcriptional Regulation.Molecular cell · 2018Article
- Timing of gene expression in a cell-fate decision system.Molecular systems biology · 2018Article
- Assigning function to natural allelic variation via dynamic modeling of gene network induction.Molecular systems biology · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein expression is a dynamic process, which can be rapidly induced by extracellular signals. It is widely appreciated that single cells can display large variations in the level of gene induction. However, the variability in the dynamics of this process in individual cells is difficult to quantify using standard fluorescent protein (FP) expression assays, due to the slow maturation of their fluorophore. Here we have developed expression reporters that accurately measure both the levels and dynamics of protein synthesis in live single cells with a temporal resolution under a minute. Our system relies on the quantification of the translocation of a constitutively expressed FP into the nucleus. As a proof of concept, we used these reporters to measure the transient protein synthesis arising from two promoters responding to the yeast hyper osmolarity glycerol mitogen-activated protein kinase pathway (pSTL1 and pGPD1). They display distinct expression dynamics giving rise to strikingly different instantaneous expression noise.
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