Evidence map›Paper›PMID 27096707›Full record

ArticleCell cycle (Georgetown, Tex.)2016

PP2A regulates kinetochore-microtubule attachment during meiosis I in oocyte.

An Tang, Peiliang Shi, Anying Song, Dayuan Zou, Yue Zhou, Pengyu Gu, Zan Huang, Qinghua Wang, Zhaoyu Lin, Xiang Gao

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell cycle (Georgetown, Tex.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 30 citations in OpenAlex.

  1. Targeting PP2A in cancer: an underrated option.Journal of experimental & clinical cancer research : CR · 2025
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Review
  9. Review
  10. Article
  11. Review
  12. Article
  13. PP2A in meiotic oocytes.Cell cycle (Georgetown, Tex.) · 2016
    Article
  14. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

An Tanga State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing Biomedical Research Institute, Collaborative Innovation Center of Genetics and Development, Nanjing University , Nanjing , China.
Peiliang Shia State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing Biomedical Research Institute, Collaborative Innovation Center of Genetics and Development, Nanjing University , Nanjing , China.
Anying Songa State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing Biomedical Research Institute, Collaborative Innovation Center of Genetics and Development, Nanjing University , Nanjing , China.
Dayuan Zoua State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing Biomedical Research Institute, Collaborative Innovation Center of Genetics and Development, Nanjing University , Nanjing , China.
Yue Zhoub State Key Laboratory of Food Science and Technology, Jiangnan University , Wuxi , China.
Pengyu Guc Neurobiology Department , University of Massachusetts Medical School , Worcester , MA , USA.
Zan Huangd College of Animal Science & Technology, Nanjing Agricultural University , Nanjing , China.
Qinghua Wanga State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing Biomedical Research Institute, Collaborative Innovation Center of Genetics and Development, Nanjing University , Nanjing , China.
Zhaoyu Lina State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing Biomedical Research Institute, Collaborative Innovation Center of Genetics and Development, Nanjing University , Nanjing , China.
Xiang Gaoa State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing Biomedical Research Institute, Collaborative Innovation Center of Genetics and Development, Nanjing University , Nanjing , China.
Model Animal Research Center · CNNanjing University · CNJiangnan University · CNNanjing Agricultural University · CNUniversity of Massachusetts Chan Medical School · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Studies using in vitro cultured oocytes have indicated that the protein phosphatase 2A (PP2A), a major serine/threonine protein phosphatase, participates in multiple steps of meiosis. Details of oocyte maturation regulation by PP2A remain unclear and an in vivo model can provide more convincing information. Here, we inactivated PP2A by mutating genes encoding for its catalytic subunits (PP2Acs) in mouse oocytes. We found that eliminating both PP2Acs caused female infertility. Oocytes lacking PP2Acs failed to complete 1(st) meiotic division due to chromosome misalignment and abnormal spindle assembly. In mitosis, PP2A counteracts Aurora kinase B/C (AurkB/C) to facilitate correct kinetochore-microtubule (KT-MT) attachment. In meiosis I in oocyte, we found that PP2Ac deficiency destabilized KT-MT attachments. Chemical inhibition of AurkB/C in PP2Ac-null oocytes partly restored the formation of lateral/merotelic KT-MT attachments but not correct KT-MT attachments. Taken together, our findings demonstrate that PP2Acs are essential for chromosome alignments and regulate the formation of correct KT-MT attachments in meiosis I in oocytes.

Indexed as

MeiosisAmino Acid SequenceAnimalsAurora Kinase BAurora Kinase CChromosomes, MammalianFemaleGene Expression Regulation, DevelopmentalInfertility, FemaleKinetochoresMiceMice, TransgenicMicrotubulesMitosisOocytesPrimary Cell CultureAURKB protein, humanAURKC protein, humanAurora Kinase BAurora Kinase CProtein Phosphatase 2Protein SubunitsfertilityKT-MT attachmentmeiosisoocytePP2A

Identifiers

PMID27096707
PMCPMC4934050
OpenAlexW2338403687

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.