ArticleEquine veterinary journal2017
Comparative transcriptome analysis of equine alveolar macrophages.
Article in Equine veterinary journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Systematic establishment of approaches to the detection of equine macrophage polarization and their application in pathogenic infection.Microbiology spectrum · 2026Article
- Equine models in translational medicine: A comparative approach to human health.Animal models and experimental medicine · 2026Review
- RNA-seq evaluation of equine alveolar macrophages and monocyte-derived macrophages exposed to an inflammatory stimulus (short communication).BMC veterinary research · 2026Article
- Targeting Toll-like Receptor 2: synthetic diacylated lipopeptides polarize equine macrophages towards a pro-inflammatory phenotype.Frontiers in immunology · 2026Article
- Equine monocyte-derived macrophages revisited: isolation and comprehensive characterization of pro- versus anti-inflammatory polarisation.The veterinary quarterly · 2025Article
- Equine alveolar macrophages and monocyte-derived macrophages respond differently to an inflammatory stimulus.PloS one · 2023Article
- The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing.Animals : an open access journal from MDPI · 2022Review
- Science-in-brief: Genomic and transcriptomic approaches to the investigation of equine diseases.Equine veterinary journal · 2022Article
- Single-cell gene expression analysis of cryopreserved equine bronchoalveolar cells.Frontiers in immunology · 2022Article
- Role of Innate Immunity in Initiation and Progression of Osteoarthritis, with Emphasis on Horses.Animals : an open access journal from MDPI · 2021Review
- Bone marrow mononuclear cells for joint therapy: The role of macrophages in inflammation resolution and tissue repair.World journal of stem cells · 2021Review
- Application across species of a one health approach to liquid sample handling for respiratory based -omics analysis.Scientific reports · 2021Article
- Transcriptional and Histochemical Signatures of Bone Marrow Mononuclear Cell-Mediated Resolution of Synovitis.Frontiers in immunology · 2021Article
- Alveolar macrophage phenotypes in severe equine asthma.Veterinary journal (London, England : 1997) · 2020Article
- Species-Specificity of Transcriptional Regulation and the Response to Lipopolysaccharide in Mammalian Macrophages.Frontiers in cell and developmental biology · 2020Article
- Acute systemic inflammatory response to lipopolysaccharide stimulation in pigs divergently selected for residual feed intake.BMC genomics · 2019Article
- Evaluation of amniotic mesenchymal cell derivatives on cytokine production in equine alveolar macrophages: an in vitro approach to lung inflammation.Stem cell research & therapy · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
reasons for performing studyAlveolar macrophages (AMs) are the first line of defence against pathogens in the lungs of all mammalian species and thus may constitute appropriate therapeutic target cells in the treatment and prevention of opportunistic airway infections. Therefore, acquiring a better understanding of equine macrophage biology is of paramount importance in addressing this issue in relation to the horse.
objectivesTo compare the transcriptome of equine AMs with that of equine peritoneal macrophages (PMs) and to investigate the effect of lipopolysaccharide (LPS) on equine AM. STUDY
designGene expression study of equine AMs.
methodsCells from both bronchoalveolar and peritoneal lavage fluid were isolated from systemically healthy horses that had been submitted to euthanasia. Cells were cryopreserved. RNA was extracted and comparative microarray analyses were performed in AMs and PMs, and in AMs treated and untreated with LPS. Comparisons with published data derived from human AM studies were made, with particular focus on LPS-induced inflammatory status.
resultsThe comparison between AMs and PMs revealed the differential basal expression of 451 genes. Gene expression analysis revealed an alternative (M2) macrophage polarisation profile in AMs and a hybrid macrophage activation profile in PMs, a phenomenon potentially attributable to a degree of induced endotoxin tolerance. The gene expression profile of equine AMs following LPS stimulation revealed significant changes in the expression of 240 genes, including well-known upregulated inflammatory genes. This LPS-induced gene expression profile of equine AMs more closely resembles that of human rather than murine macrophages.
conclusionsThis study improves current understanding of equine macrophage biology. These data suggest that the horse may represent a suitable animal model for the study of human macrophage-associated lung inflammation and data derived from human macrophage studies may have significant relevance to the horse.
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