ArticleIranian journal of parasitology
Genetic Characterization of Fasciola Isolates from West Azerbaijan Province Iran Based on ITS1 and ITS2 Sequence of Ribosomal DNA.
Article in Iranian journal of parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Seroprevalence of Fascioliasis in Patients Referred to the Reference Laboratory in Urmia, Northwest Iran.Journal of parasitology research · 2026Article
- Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of Iran.Veterinary medicine and science · 2024Article
- Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran.Veterinary medicine and science · 2023Article
- Molecular Characterization ofInfection and drug resistance · 2023Article
- Molecular Characterization and Immunodiagnostic Potential of Various Antigenic Proteins ofHelminthologia · 2019Article
- Fascioliasis mimicking malignancy in an old patient.BioMedicine · 2019Article
- Molecular characterization ofSaudi journal of biological sciences · 2019Article
- Molecular characterization ofVeterinary world · 2016Article
- Prevalence of Animal Fasciolosis and Specification ofIranian journal of parasitologyArticle
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Abstract
backgroundFascioliasis, caused by Fasciola hepatica and F. gigantica, has medical and economic importance in the world. Molecular approaches comparing traditional methods using for identification and characterization of Fasciola spp. are precise and reliable. The aims of current study were molecular characterization of Fasciola spp. in West Azerbaijan Province, Iran and then comparative analysis of them using GenBank sequences.
methodsA total number of 580 isolates were collected from different hosts in five cities of West Azerbaijan Province, in 2014 from 90 slaughtered cattle (n=50) and sheep (n=40). After morphological identification and DNA extraction, designing specific primer were used to amplification of ITS1, 5.8s and ITS2 regions, 50 samples were conducted to sequence, randomly.
resultUsing morphometric characters 99.14% and 0.86% of isolates identified as F. hepatica and F. gigantica, respectively. PCR amplification of 1081 bp fragment and sequencing result showed 100% similarity with F. hepatica in ITS1 (428 bp), 5.8s (158 bp), and ITS2 (366 bp) regions. Sequence comparison among current study sequences and GenBank data showed 98% identity with 11 nucleotide mismatches. However, in phylogenetic tree F. hepatica sequences of West Azerbaijan Province, Iran, were in a close relationship with Iranian, Asian, and African isolates.
conclusionsOnly F. hepatica species is distributed among sheep and cattle in West Azerbaijan Province Iran. However, 5 and 6 bp variation in ITS1 and ITS2 regions, respectively, is not enough to separate of Fasciola spp. Therefore, more studies are essential for designing new molecular markers to correct species identification.
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