ArticleThe Plant cell2016
Pollen-Specific Aquaporins NIP4;1 and NIP4;2 Are Required for Pollen Development and Pollination in Arabidopsis thaliana.
Article in The Plant cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 105 citations in OpenAlex.
- Cork oak aquaporins: functional diversity and regulation with insights into drought response.Plant cell reports · 2026Article
- Transcriptional and post-translational regulation of aquaporins in plants.Stress biology · 2026Review
- Genome-wide identification of aquaporin family inFrontiers in plant science · 2026Article
- Transcriptomic responses of Antarctic plants to in situ warming: uncovering molecular mechanisms behind physiological adjustments.Annals of botany · 2025Article
- From lock and key to molecular diplomacy: understanding pollen recognition and discrimination in brassicaceae.Plant reproduction · 2024Review
- Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement.Plant reproduction · 2024Review
- Physiological roles of Arabidopsis MCA1 and MCA2 based on their dynamic expression patterns.Journal of plant research · 2024Review
- Boron homeostasis affects Longan yield: a study of NIP and BOR boron transporter of two cultivars.BMC plant biology · 2024Article
- Analogy of silicon and boron in plant nutrition.Frontiers in plant science · 2024Review
- Stop and go signals at the stigma-pollen interface of the Brassicaceae.Plant physiology · 2023Article
- Beyond NPK: Mineral Nutrient-Mediated Modulation in Orchestrating Flowering Time.Plants (Basel, Switzerland) · 2023Review
- What Can Boron Deficiency Symptoms Tell Us about Its Function and Regulation?Plants (Basel, Switzerland) · 2023Review
- Genome-wide identification and biological relevance of broccoli aquaporins.The plant genome · 2022Article
- The structure, function and expression analysis of the nodulin 26-like intrinsic protein subfamily of plant aquaporins in tomato.Scientific reports · 2022Article
- Calmodulin Domain Protein Kinase PiCDPK1 Regulates Pollen Tube Growth Polarity through Interaction with RhoGDI.Plants (Basel, Switzerland) · 2022Article
- The role of CDPKs in plant development, nutrient and stress signaling.Frontiers in genetics · 2022Review
- Metalloid transporters and their regulation in plants.Plant physiology · 2021Review
- Calcium-dependent protein kinase 29 modulates PIN-FORMED polarity and Arabidopsis development via its own phosphorylation code.The Plant cell · 2021Article
- Citrus NIP5;1 aquaporin regulates cell membrane water permeability and alters PIPs plasma membrane localization.Plant molecular biology · 2021Article
- Two aquaporins, SIP1;1 and PIP1;2, mediate water transport for pollen hydration in thePlant biotechnology (Tokyo, Japan) · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In flowers with dry stigmas, pollen development, pollination, and pollen tube growth require spatial and temporal regulation of water and nutrient transport. To better understand the molecular mechanisms involved in reproductive processes, we characterized NIP4;1 and NIP4;2, two pollen-specific aquaporins of Arabidopsis thaliana. NIP4;1 and NIP4;2 are paralogs found exclusively in the angiosperm lineage. Although they have 84% amino acid identity, they displayed different expression patterns. NIP4;1 has low expression levels in mature pollen, while NIP4;2 expression peaks during pollen tube growth. Additionally, NIP4;1pro:GUS flowers showed GUS activity in mature pollen and pollen tubes, whereas NIP4;2pro:GUS flowers only in pollen tubes. Single T-DNA mutants and double artificial microRNA knockdowns had fewer seeds per silique and reduced pollen germination and pollen tube length. Transport assays in oocytes showed NIP4;1 and NIP4;2 function as water and nonionic channels. We also found that NIP4;1 and NIP4;2 C termini are phosphorylated by a pollen-specific CPK that modifies their water permeability. Survival assays in yeast indicated that NIP4;1 also transports ammonia, urea, boric acid, and H2O2 Thus, we propose that aquaporins NIP4;1 and NIP4;2 are exclusive components of the reproductive apparatus of angiosperms with partially redundant roles in pollen development and pollination.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.