ArticleScientific reports2016
Testing the causality between CYP9M10 and pyrethroid resistance using the TALEN and CRISPR/Cas9 technologies.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 86 citations in OpenAlex.
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- Overexpression of multiple cytochrome P450 genes with and without knockdown resistance mutations confers high resistance to deltamethrin in Culex quinquefasciatus.Infectious diseases of poverty · 2025Article
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
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- CRISPR-mediated knock-in of transgenes into the malaria vector Anopheles funestus.G3 (Bethesda, Md.) · 2021Article
- Evaluation of Gene Knockouts by CRISPR as Potential Targets for the Genetic Engineering of the MosquitoThe CRISPR journal · 2021Article
- CRISPR/Cas-9 mediated knock-in by homology dependent repair in the West Nile Virus vector Culex quinquefasciatus Say.Scientific reports · 2021Article
- A first, naturally occurring substitution at the second pyrethroid receptor of voltage-gated sodium channel of Aedes aegypti.Pest management science · 2021Article
- Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes.Nature communications · 2021Article
- Multiple cytochrome P450 genes: conferring high levels of permethrin resistance in mosquitoes, Culex quinquefasciatus.Scientific reports · 2021Article
- From Global to Local-New Insights into Features of Pyrethroid Detoxification in Vector Mosquitoes.Insects · 2021Review
- The Central Role of Multiple P450 Genes and Their Co-factor CPR in the Development of Permethrin Resistance in the MosquitoFrontiers in physiology · 2021Article
- Can CRISPR gene drive work in pest and beneficial haplodiploid species?Evolutionary applications · 2020Article
- CRISPR-Mediated Knockout of theToxins · 2020Article
- Mosquito antiviral defense mechanisms: a delicate balance between innate immunity and persistent viral infection.Parasites & vectors · 2019Review
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- Vector biology meets disease control: using basic research to fight vector-borne diseases.Nature microbiology · 2019Review
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- A perspective on the need and current status of efficient sex separation methods for mosquito genetic control.Parasites & vectors · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently-emerging genome editing technologies have enabled targeted gene knockout experiments even in non-model insect species. For studies on insecticide resistance, genome editing technologies offer some advantages over the conventional reverse genetic technique, RNA interference, for testing causal relationships between genes of detoxifying enzymes and resistance phenotypes. There were relatively abundant evidences indicating that the overexpression of a cytochrome P450 gene CYP9M10 confers strong pyrethroid resistance in larvae of the southern house mosquito Culex quinquefasciatus. However, reverse genetic verification has not yet been obtained because of the technical difficulty of microinjection into larvae. Here, we tested two genome editing technologies, transcription activator-like effector nucleases (TALEN)s and clustered regularly interspaced short palindromic repeats (CRISPR/Cas9), to disrupt CYP9M10 in a resistant strain of C. quinquefasciatus. Additionally, we developed a novel, effective approach to construct a TALE using the chemical cleavage of phosphorothioate inter-nucleotide linkages in the level 1 assembly. Both TALEN and CRISPR/Cas9 induced frame-shifting mutations in one or all copies of CYP9M10 in a pyrethroid-resistant strain. A line fixed with a completely disrupted CYP9M10 haplotype showed more than 100-fold reduction in pyrethroid resistance in the larval stage.
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