Evidence map›Paper›PMID 27095569›Full record

ArticleParasitology research2016

An easy 'one tube' method to estimate viability of Cryptosporidium oocysts using real-time qPCR.

A Paziewska-Harris, G Schoone, H D F H Schallig

Abstract read
PubMed Publisher
In one paragraph

Article in Parasitology research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

A Paziewska-HarrisRoyal Tropical Institute, Parasitology Unit, KIT Biomedical Research, Meibergdreef 39, 1105 AZ, Amsterdam, The Netherlands. a.paziewska@kit.nl.
G SchooneRoyal Tropical Institute, Parasitology Unit, KIT Biomedical Research, Meibergdreef 39, 1105 AZ, Amsterdam, The Netherlands.
H D F H SchalligRoyal Tropical Institute, Parasitology Unit, KIT Biomedical Research, Meibergdreef 39, 1105 AZ, Amsterdam, The Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Viability estimation of the highly resistant oocysts of Cryptosporidium remains a key issue for the monitoring and control of this pathogen. We present here a simple 'one tube' quantitative PCR (qPCR) protocol for viability estimation using a DNA extraction protocol which preferentially solubilizes excysted sporozoites rather than oocysts. Parasite DNA released from excysted sporozoites was quantified by real-time qPCR using a ribosomal DNA marker. The qPCR signal was directly proportional to the number of oocysts excysted, and a power-law relationship was noted between oocyst age and the proportion excysting. Unexcysted oocysts released negligible amounts of DNA making the method suitable for estimating viability of as few as 10 oocysts.

Indexed as

AnimalsBiomarkersCryptosporidiosisCryptosporidium parvumDNA, ProtozoanDNA, RibosomalOocystsReal-Time Polymerase Chain ReactionSporozoitesBiomarkersDNA, ProtozoanDNA, RibosomalCryptosporidium parvumEnvironmental samplesExcystationSporozoitesWater quality monitoring

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.