ArticleScientific reports2016
Ablation of the androgen receptor from vascular smooth muscle cells demonstrates a role for testosterone in vascular calcification.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
42 citing papers in PubMed, 76 citations in OpenAlex.
- Reduction of calprotectin and phosphate during testosterone therapy in aging men: a randomized controlled trial.Journal of endocrinological investigation · 2017Trial
- Cardiovascular Disease and Androgens: Clinical Trends, Potential Mechanisms, and Considerations for Engineering Solutions.Cardiovascular engineering and technology · 2026Review
- The potential role of sex related differences of the microbiome and its impact on calcific aortic stenosis: a narrative review.Biology of sex differences · 2026Review
- The Role of Testosterone in Atherosclerosis: View From Cell Cultures and Animal Models.Journal of cardiovascular translational research · 2026Review
- Review
- Aortic Valve Calcium: A Narrative Review of its Role in the Assessment of Aortic Stenosis and as a Predictor of Post-transcatheter Aortic Valve Implantation Outcomes.Interventional cardiology (London, England) · 2025Review
- Hormonal influence: unraveling the impact of sex hormones on vascular smooth muscle cells.Biological research · 2024Review
- Review
- The Spectrum of Coronary Artery Disease in Elite Endurance Athletes-A Long-Standing Debate: State-of-the-Art Review.Journal of clinical medicine · 2024Review
- Effects of Sex Hormones on Vascular Reactivity in Boys With Hypospadias.The Journal of clinical endocrinology and metabolism · 2024Article
- Serum DHEA and Testosterone Levels Associate Inversely With Coronary Artery Calcification in Elderly Men.The Journal of clinical endocrinology and metabolism · 2023Article
- Alerting trends in epidemiology for calcific aortic valve disease, 1990-2019: An age-period-cohort analysis for the Global Burden of Disease Study 2019.European heart journal. Quality of care & clinical outcomes · 2023Article
- Potential mechanisms linking high-volume exercise with coronary artery calcification.Heart (British Cardiac Society) · 2023Review
- Epigenome-Wide Changes in the Cell Layers of the Vein Wall When Exposing the Venous Endothelium to Oscillatory Shear Stress.Epigenomes · 2023Article
- The Molecular Signature of Human Testicular Peritubular Cells Revealed by Single-Cell Analysis.Cells · 2022Article
- Impact of sex and sex hormones on pathophysiology and progression of aortic stenosis in a murine model.Physiological reports · 2022Article
- Review
- Sex Differences and Similarities in Valvular Heart Disease.Circulation research · 2022Review
- Menopause, androgens, and cardiovascular ageing: a narrative review.Therapeutic advances in endocrinology and metabolism · 2022Review
- Sex Steroid Regulation of Oxidative Stress in Bone Cells: An In Vitro Study.International journal of environmental research and public health · 2021Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Vascular calcification powerfully predicts mortality and morbidity from cardiovascular disease. Men have a greater risk of cardiovascular disease, compared to women of a similar age. These gender disparities suggest an influence of sex hormones. Testosterone is the primary and most well-recognised androgen in men. Therefore, we addressed the hypothesis that exogenous androgen treatment induces vascular calcification. Immunohistochemical analysis revealed expression of androgen receptor (AR) in the calcified media of human femoral artery tissue and calcified human valves. Furthermore, in vitro studies revealed increased phosphate (Pi)-induced mouse vascular smooth muscle cell (VSMC) calcification following either testosterone or dihydrotestosterone (DHT) treatment for 9 days. Testosterone and DHT treatment increased tissue non-specific alkaline phosphatase (Alpl) mRNA expression. Testosterone-induced calcification was blunted in VSMC-specific AR-ablated (SM-ARKO) VSMCs compared to WT. Consistent with these data, SM-ARKO VSMCs showed a reduction in Osterix mRNA expression. However, intriguingly, a counter-intuitive increase in Alpl was observed. These novel data demonstrate that androgens play a role in inducing vascular calcification through the AR. Androgen signalling may represent a novel potential therapeutic target for clinical intervention.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.