ArticleRNA (New York, N.Y.)2016
The local expression and trafficking of tyrosine hydroxylase mRNA in the axons of sympathetic neurons.
Article in RNA (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- Tyrosine Hydroxylase Deficiency Impairs TH Axonal Transport, Brain Function, and Neuronal Plasticity.Journal of inherited metabolic disease · 2026Article
- RNAi-based screen for pigmentation iniScience · 2026Article
- Retrograde control of sympathetic neuron-satellite glia interactions by target-derived NGF signaling.Cell reports · 2025Article
- RNAi-based screen for pigmentation inbioRxiv : the preprint server for biology · 2025Article
- Dopamine release and dopamine-related gene expression in the amygdala are modulated by the gastrin-releasing peptide in opposite directions during stress-enhanced fear learning and extinction.Molecular psychiatry · 2025Article
- Monocyte-Derived Macrophages Aggravate Cardiac Dysfunction After Ischemic Stroke in Mice.Journal of the American Heart Association · 2024Article
- Electroacupuncture attenuates myocardial ischemia-reperfusion injury by inhibiting microglial engulfment of dendritic spines.iScience · 2023Article
- The motivation to flock correlates with vocal-social behaviors and dopamine-related gene expression in male European starlings.Hormones and behavior · 2023Article
- Neuro-mesodermal assembloids recapitulate aspects of peripheral nervous system development in vitro.Stem cell reports · 2023Article
- CSF1R-Mediated Myeloid Cell Depletion Prolongs Lifespan But Aggravates Distinct Motor Symptoms in a Model of Multiple System Atrophy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Examining the role of paraoxonase 2 in the dopaminergic system of the mouse brain.BMC neuroscience · 2022Article
- Article
- Olfactory regulation by dopamine and DRD2 receptor in the nose.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- An immune-sympathetic neuron communication axis guides adipose tissue browning in cancer-associated cachexia.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Subcellular and regional localization of mRNA translation in midbrain dopamine neurons.Cell reports · 2022Article
- Nicotine preference and affective behavior of Cd81 knockout mice.Psychopharmacology · 2021Article
- Methylation of the tyrosine hydroxylase gene is dysregulated by cocaine dependence in the human striatum.iScience · 2021Article
- Late onset of Synaptotagmin 2a expression at synapses relevant to social behavior.The Journal of comparative neurology · 2021Article
- Alterations of Dopamine-Related Transcripts in A11 Diencephalospinal Pathways after Spinal Cord Injury.Neural plasticity · 2021Article
- Molecular mechanisms behind mRNA localization in axons.Open biology · 2020Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Synthesis and regulation of catecholamine neurotransmitters in the central nervous system are implicated in the pathogenesis of a number of neuropsychiatric disorders. To identify factors that regulate the presynaptic synthesis of catecholamines, we tested the hypothesis that the rate-limiting enzyme of the catecholamine biosynthetic pathway, tyrosine hydroxylase (TH), is locally synthesized in axons and presynaptic nerve terminals of noradrenergic neurons. To isolate pure axonal mRNA and protein, rat superior cervical ganglion sympathetic neurons were cultured in compartmentalized Campenot chambers. qRT-PCR and RNA in situ hybridization analyses showed that TH mRNA is present in distal axons. Colocalization experiments with nerve terminal marker proteins suggested that both TH mRNA and protein localize in regions of the axon that resemble nerve terminals (i.e., synaptic boutons). Analysis of polysome-bound RNA showed that TH mRNA is present in polysomes isolated from distal axons. Metabolic labeling of axonally synthesized proteins labeled with the methionine analog, L-azidohomoalanine, showed that TH is locally synthesized in axons. Moreover, the local transfection and translation of exogenous TH mRNA into distal axons facilitated axonal dopamine synthesis. Finally, using chimeric td-Tomato-tagged constructs, we identified a sequence element within the TH 3'UTR that is required for the axonal localization of the reporter mRNA. Taken together, our results provide the first direct evidence that TH mRNA is trafficked to the axon and that the mRNA is locally translated. These findings raise the interesting possibility that the biosynthesis of the catecholamine neurotransmitters is locally regulated in the axon and/or presynaptic nerve terminal.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.