ArticleInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases2016
Human norovirus hyper-mutation revealed by ultra-deep sequencing.
Article in Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Evaluation of ultraviolet irradiation at 254 nm and 222 nm in inactivating human noroviruses on surfaces.Applied and environmental microbiology · 2026Article
- An unusual diarrheal outbreak in the community in Eastern Thailand caused by Norovirus GII.3[P25].Virology journal · 2024Article
- Inactivation of Foodborne Viruses by UV Light: A Review.Foods (Basel, Switzerland) · 2021Review
- ADAR Editing in Viruses: An Evolutionary Force to Reckon with.Genome biology and evolution · 2021Review
- Review
- Novel and emerging mutations of SARS-CoV-2: Biomedical implications.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2021Review
- Article
- High Throughput Sequencing for the Detection and Characterization of RNA Viruses.Frontiers in microbiology · 2021Review
- Point mutation bias in SARS-CoV-2 variants results in increased ability to stimulate inflammatory responses.Scientific reports · 2020Article
- A-to-I editing of Malacoherpesviridae RNAs supports the antiviral role of ADAR1 in mollusks.BMC evolutionary biology · 2019Article
- Transmission routes of rare seasonal diseases: the case of norovirus infections.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- A survey on cellular RNA editing activity in response to Candida albicans infections.BMC genomics · 2018Article
- A comprehensive study on cellular RNA editing activity in response to infections with different subtypes of influenza a viruses.BMC genomics · 2018Article
- Characterization of intra- and inter-host norovirus P2 genetic variability in linked individuals by amplicon sequencing.PloS one · 2018Article
- Static and Evolving Norovirus Genotypes: Implications for Epidemiology and Immunity.PLoS pathogens · 2017Article
- Mutational Pressure in Zika Virus: Local ADAR-Editing Areas Associated with Pauses in Translation and Replication.Frontiers in cellular and infection microbiology · 2017Article
- Mechanisms of viral mutation.Cellular and molecular life sciences : CMLS · 2016Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human noroviruses (NoVs) are a major cause of gastroenteritis worldwide. It is thought that, similar to other RNA viruses, high mutation rates allow NoVs to evolve fast and to undergo rapid immune escape at the population level. However, the rate and spectrum of spontaneous mutations of human NoVs have not been quantified previously. Here, we analyzed the intra-patient diversity of the NoV capsid by carrying out RT-PCR and ultra-deep sequencing with 100,000-fold coverage of 16 stool samples from symptomatic patients. This revealed the presence of low-frequency sequences carrying large numbers of U-to-C or A-to-G base transitions, suggesting a role for hyper-mutation in NoV diversity. To more directly test for hyper-mutation, we performed transfection assays in which the production of mutations was restricted to a single cell infection cycle. This confirmed the presence of sequences with multiple U-to-C/A-to-G transitions, and suggested that hyper-mutation contributed a large fraction of the total NoV spontaneous mutation rate. The type of changes produced and their sequence context are compatible with ADAR-mediated editing of the viral RNA.
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