ArticlePloS one2016
Transgenic Sugarcane with a cry1Ac Gene Exhibited Better Phenotypic Traits and Enhanced Resistance against Sugarcane Borer.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Revisiting the Molecular Roadmap for Sugar Crops: Genome Reading, Trait Writing and Variety Redesigning.Plant biotechnology journal · 2026Review
- Cry1Ab toxicity against four species of the Diatraea sugarcane borer complex in Colombia.Scientific reports · 2026Article
- Sugarcane Breeding in the Genomic Era: Integrative Strategies and Emerging Technologies.Plants (Basel, Switzerland) · 2026Review
- Transgenic sugarcane harboringFrontiers in plant science · 2026Article
- Enhancing quality and climate resilient traits in vegetatively propagated polyploids: transgenic and genome editing advancements, challenges and future directions.Frontiers in genetics · 2025Review
- Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses.Plants (Basel, Switzerland) · 2024Review
- Factors affecting the production of sugarcane yield and sucrose accumulation: suggested potential biological solutions.Frontiers in plant science · 2024Review
- Integrating the Soil Microbiota and Metabolome Reveals the Mechanism through Which Controlled Release Fertilizer Affects Sugarcane Growth.International journal of molecular sciences · 2023Article
- Technological Development and Application of Plant Genetic Transformation.International journal of molecular sciences · 2023Review
- New methods for sorghum transformation in temperate climates.AoB PLANTS · 2023Review
- Correlation of microbiomes in "plant-insect-soil" ecosystem.Frontiers in microbiology · 2023Article
- Article
- Developing new sugarcane varieties suitable for mechanized production in China: principles, strategies and prospects.Frontiers in plant science · 2023Article
- Genetic engineering: an efficient approach to mitigating biotic and abiotic stresses in sugarcane cultivation.Plant signaling & behavior · 2022Article
- A highly efficient stratagem for protoplast isolation and genetic transformation in filamentous fungus Colletotrichum falcatum.Folia microbiologica · 2022Article
- Genetic Engineering Approaches for Enhanced Insect Pest Resistance in Sugarcane.Molecular biotechnology · 2021Review
- hRNAi-mediated knock-down of Sphenophorus levis V-ATPase E in transgenic sugarcane (Saccharum spp interspecific hybrid) affects the insect growth and survival.Plant cell reports · 2021Article
- Applying Molecular Phenotyping Tools to Explore Sugarcane Carbon Potential.Frontiers in plant science · 2021Review
- Genetic Transformation of Sugarcane, Current Status and Future Prospects.Frontiers in plant science · 2021Review
- ImprovementGM crops & food · 2021Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed sugarcane plants with improved resistance to the sugarcane borer, Diatraea saccharalis (F). An expression vector pGcry1Ac0229, harboring the cry1Ac gene and the selectable marker gene, bar, was constructed. This construct was introduced into the sugarcane cultivar FN15 by particle bombardment. Transformed plantlets were identified after selection with Phosphinothricin (PPT) and Basta. Plantlets were then screened by PCR based on the presence of cry1Ac and 14 cry1Ac positive plantlets were identified. Real-time quantitative PCR (RT-qPCR) revealed that the copy number of cry1Ac gene in the transgenic lines varied from 1 to 148. ELISA analysis showed that Cry1Ac protein levels in 7 transgenic lines ranged from 0.85 μg/FWg to 70.92 μg/FWg in leaves and 0.04 μg/FWg to 7.22 μg/FWg in stems, and negatively correlated to the rate of insect damage that ranged from 36.67% to 13.33%, respectively. Agronomic traits of six transgenic sugarcane lines with medium copy numbers were similar to the non-transgenic parental line. However, phenotype was poor in lines with high or low copy numbers. Compared to the non-transgenic control plants, all transgenic lines with medium copy numbers had relatively equal or lower sucrose yield and significantly improved sugarcane borer resistance, which lowered susceptibility to damage by insects. This suggests that the transgenic sugarcane lines harboring medium copy numbers of the cry1Ac gene may have significantly higher resistance to sugarcane borer but the sugarcane yield in these lines is similar to the non-transgenic control thus making them superior to the control lines.
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