Evidence map›Paper›PMID 27092218›Full record

ArticleIranian journal of microbiology2016

A bioassay-guided fractionation scheme for characterization of new antibacterial compounds from Prosopis cineraria aerial parts.

Mahdieh Neghabi-Hajiagha, Atousa Aliahmadi, Mohammad Reza Taheri, Alireza Ghassempour, Gholamreza Irajian, Hassan Rezadoost, Mohammad Mehdi Feizabadi

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In one paragraph

Article in Iranian journal of microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. In-Silico Validation ofInternational journal of peptide research and therapeutics · 2022
    Article
  2. Review
  3. Biomolecules · 2019
    Review
  4. Comparative Evaluation ofFrontiers in pharmacology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mahdieh Neghabi-HajiaghaDepartment of Microbiology, School of Medicine, Tehran University of Medical Sciences, Poorsina street, Tehran, Iran.
Atousa AliahmadiDepartment of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University.
Mohammad Reza TaheriDepartment of Phytochemistry, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Iran.
Alireza GhassempourDepartment of Phytochemistry, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Iran.
Gholamreza IrajianDepartment of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Hassan RezadoostDepartment of Phytochemistry, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Iran.
Mohammad Mehdi FeizabadiDepartment of Microbiology, School of Medicine, Tehran University of Medical Sciences, Poorsina street, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesDue to the importance of finding of new antibacterial agents, the antibacterial properties of Prosopis cineraria aerial parts were investigated using a bioassay guided fractionation scheme. MATERIALS AND

methodsThe organic extract was prepared via maceration in methanol, followed by the fractionation using n-hexane and ethyl acetate. The MICs of fractions were determined against some human pathogenic bacteria using broth micro-dilution assay. The primary characterization and identification of bioactive substance(s) was based on a bio-autographical method using HPTLC and flash chromatography in parallel with agar overlay assays. Finally the exact mass of effective compound(s) was determined by LC-MS.

resultsThe best antibacterial activities were related to the ethyl acetate fraction. The effective antibacterial compound of the plant were 2 substances with molecular weight of 348 and 184 Dalton that inhibited the growth of assessed Gram positive bacteria with MIC values lower than 125 to 62.5 μg/ml synergistically.

conclusionFurther analysis using nuclear magnetic resonance could reveal the exact structure of these two antibacterial substances. These 2 effective antibacterial compounds could be applied as lead compound for synthesis of new antibacterial agents.

Indexed as

Antibiotic resistantbioassaycharacterizationProsopis cinerariaStaphylococcus aureus

Identifiers

PMID27092218
PMCPMC4833735

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.