ArticleFrontiers in microbiology2016
Metataxonomic and Metagenomic Approaches vs. Culture-Based Techniques for Clinical Pathology.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
70 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Metagenomics or Metataxonomics: Best Practice Methods to Uncover the Sinus Microbiome.International forum of allergy & rhinology · 2025Guideline
- Metagenomic Analysis of Thoracic Empyema Etiology Through Next-Generation Sequencing Enhances Conventional Culture Techniques.Infection & chemotherapy · 2026Article
- Meat-Borne Bacterial Pathogen Detection: Conventional, Molecular and Emerging AI-Based Strategies.Diagnostics (Basel, Switzerland) · 2026Review
- Childhood obesity, dietary patterns and gut microbiota: a narrative review.Journal of physiology and biochemistry · 2026Review
- Fecal Microbiota Profiling in Indigenous Backyard and Commercial Chickens Reveals Distinct Taxonomic Signatures.Veterinary medicine international · 2026Article
- Hospital Air and Healthcare Personnel as Reservoirs of Drug-Resistant Bacteria in Tertiary Care Settings in Pakistan.Infection and drug resistance · 2026Article
- Metagenomic next-generation sequencing improves diagnosis ofFrontiers in medicine · 2026Article
- The Uterine Microbiota in Mares With Endometritis: Impacts of Antibiotic Treatment.Veterinary medicine international · 2026Article
- Application of MSB selective enrichment followed by amplicon MinION sequencing for multipathogen detection in smoked salmon.NPJ science of food · 2025Article
- Fungal Diversity and Interactions in the Nasal and Oral Cavities of Individuals with Allergic Rhinitis, Asthma and Healthy Controls.Microorganisms · 2025Article
- Leveraging innovative diagnostics as a tool to contain superbugs.Antonie van Leeuwenhoek · 2025Review
- Evaluating the feasibility, sensitivity, and specificity of next-generation molecular methods for pleural infection diagnosis.Microbiology spectrum · 2025Observational
- Comparison of bronchoalveolar lavage fluid mNGS and sputum culture in the clinical application of severe pneumonia.Frontiers in cellular and infection microbiology · 2025Article
- Nanopore targeted sequencing in lower respiratory infections: a retrospective study on diagnostic applications, clinical characterization, and antimicrobial guidance.Frontiers in cellular and infection microbiology · 2025Article
- MetaAll: integrative bioinformatics workflow for analysing clinical metagenomic data.Briefings in bioinformatics · 2024Article
- Comparison between 16S rRNA and shotgun sequencing in colorectal cancer, advanced colorectal lesions, and healthy human gut microbiota.BMC genomics · 2024Article
- Article
- The Effect ofJournal of neurogastroenterology and motility · 2024Article
- Systemic antibiotic treatment of cows with metritis early postpartum does not change the progression of uterine disease or the uterine microbiome at 1 month postpartum.Research square · 2024Article
- Sex Difference in the Effect ofJournal of cancer prevention · 2024Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Diagnoses that are both timely and accurate are critically important for patients with life-threatening or drug resistant infections. Technological improvements in High-Throughput Sequencing (HTS) have led to its use in pathogen detection and its application in clinical diagnoses of infectious diseases. The present study compares two HTS methods, 16S rRNA marker gene sequencing (metataxonomics) and whole metagenomic shotgun sequencing (metagenomics), in their respective abilities to match the same diagnosis as traditional culture methods (culture inference) for patients with ventilator associated pneumonia (VAP). The metagenomic analysis was able to produce the same diagnosis as culture methods at the species-level for five of the six samples, while the metataxonomic analysis was only able to produce results with the same species-level identification as culture for two of the six samples. These results indicate that metagenomic analyses have the accuracy needed for a clinical diagnostic tool, but full integration in diagnostic protocols is contingent on technological improvements to decrease turnaround time and lower costs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.