ArticleFrontiers in microbiology2016
Evaluation of the Bruker Biotyper Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry System for Identification of Clinical and Environmental Isolates of Burkholderia pseudomallei.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Current Melioidosis Diagnostic Landscape and Missed Opportunities in Biomarker Development.Diagnostics (Basel, Switzerland) · 2026Review
- Stability assessment of whole cell ELISA for Burkholderia pseudomallei antibodies detection.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Article
- Immunoassay-mass spectrometry to identifyFrontiers in cellular and infection microbiology · 2025Article
- Research on the establishment and application of protein fingerprint spectrum database ofNew microbes and new infections · 2021Article
- A multi-country study using MALDI-TOF mass spectrometry for rapid identification of Burkholderia pseudomallei.BMC microbiology · 2021Article
- Leg ulceration due to cutaneous melioidosis in a returning traveller.BMJ case reports · 2021Article
- Rapid Clinical Screening of Burkholderia pseudomallei Colonies by a Bacteriophage Tail Fiber-Based Latex Agglutination Assay.Applied and environmental microbiology · 2021Article
- Human Melioidosis.Clinical microbiology reviews · 2020Review
- The Brief Case: A Traveler's Tale-Burkholderia pseudomallei Infection in a Cystic Fibrosis Patient.Journal of clinical microbiology · 2019Article
- Methylome Characterization of Burkholderia pseudomallei Strain 982 at Single-Base Resolution.Microbiology resource announcements · 2019Article
- Case Series Study of Melioidosis, Colombia.Emerging infectious diseases · 2019Article
- Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer.Journal of clinical microbiology · 2019Article
- The first imported case of melioidosis in a patient in central China.Emerging microbes & infections · 2019Article
- Evaluation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying Burkholderia pseudomallei and Burkholderia thailandensis isolates.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- Utility of a Rapid Lateral Flow Assay To Resolve Erroneous Identification of Burkholderia pseudomallei as Burkholderia thailandensis by Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry.Journal of clinical microbiology · 2018Article
- Misidentification of Risk Group 3/Security Sensitive Biological Agents by MALDI-TOF MS in Canada: November 2015-October 2017.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2018Article
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6 authors.
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Abstract
Burkholderia pseudomallei is not represented in the current version of Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system. A total of 66 isolates of B. pseudomallei, including 30 clinical isolates collected from National Taiwan University Hospital (NTUH, n = 27) and Peking Union Medical College Hospital (PUMCH, n = 3), and 36 isolates of genetically confirmed strains, including 13 from clinical samples and 23 from environmental samples, collected from southern Taiwan were included in this study. All these isolates were identified by partial 16S rDNA gene sequencing analysis and the Bruker Biotyper MALDI-TOF MS system. Among the 30 isolates initially identified as B. pseudomallei by conventional identification methods, one was identified as B. cepacia complex (NTUH) and three were identified as B. putida (PUMCH) by partial 16S rDNA gene sequencing analysis and Bruker Biotyper MALDI-TOF MS system. The Bruker Biotyper MALDI-TOF MS system misidentified 62 genetically confirmed B. pseudomallei isolates as B. thailandensis or Burkholderia species (score values, 1.803-2.063) when the currently available database (DB 5627) was used. However, using a newly created MALDI-TOF MS database (including B. pseudomallei NTUH-3 strain), all isolates were correctly identified as B. pseudomallei (score values >2.000, 100%). An additional 60 isolates of genetically confirmed B. cepacia complex and B. putida were also evaluated by the Bruker Biotyper MALDI-TOF MS system using the newly created database and none of these isolates were identified as B. pseudomallei. MALDI-TOF MS is a versatile and robust tool for the rapid identification of B. pseudomallei using the enhanced database.
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