ArticleProceedings of the National Academy of Sciences of the United States of America2016
Very-long-chain fatty acids restrict regeneration capacity by confining pericycle competence for callus formation in Arabidopsis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 125 citations in OpenAlex.
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- A single-nucleus atlas of ligular region uncovers brassinosteroid-driven cell fate transitions controlling leaf angle in maize.Nature communications · 2026Article
- A VLCFA-auxin regulatory module modulates root morphogenesis in apple.Horticulture research · 2026Article
- A precise splice: proper splicing patterns required for appropriate lipid metabolism during callus induction with cytokinin.Plant physiology · 2026Article
- FERONIA defines intact tissue boundaries through cuticle development.Nature communications · 2026Article
- Stem cell activity is linked to a transient acquisition of pluripotency during callus proliferation.Nature communications · 2026Article
- Pre-mRNA splicing regulates cellular dedifferentiation via lipid metabolism in a cytokinin-dependent manner in Arabidopsis.Plant physiology · 2026Article
- Phospholipid Networks as Metabolic Hubs and Signaling Integrators in Plant Development and Stress Adaptation.Plants (Basel, Switzerland) · 2026Review
- Methylome and Transcriptome Analysis Reveals Differences in Callus Development and Plantlet Regeneration Capacity Between TwoPlants (Basel, Switzerland) · 2026Article
- Understanding Callus Types in Maize by Genetic Mapping and Transcriptional Profiling.Plants (Basel, Switzerland) · 2025Article
- Single-cell RNA sequencing reveals developmental trajectories and environmental regulation of callus formation in Arabidopsis.Stress biology · 2025Article
- The Novel Flax Cultivar Silesia Shows High Morphogenetic Capacity in Tissue Cultures.International journal of molecular sciences · 2025Article
- Response of hidden architects to salt stress.Planta · 2025Review
- Biosynthesis of very Long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development.The Plant journal : for cell and molecular biology · 2025Article
- Insights into the Molecular Basis of Pollen Coat Development and Its Role in Male Sterility.International journal of molecular sciences · 2025Review
- A single-cell transcriptome atlas reveals the trajectory of early cell fate transition during callus induction in Arabidopsis.Plant communications · 2024Article
- A point mutation in thePhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Cotton BLH1 and KNOX6 antagonistically modulate fiber elongation via regulation of linolenic acid biosynthesis.Plant communications · 2024Article
- Plant regeneration in the new era: from molecular mechanisms to biotechnology applications.Science China. Life sciences · 2024Review
- A fatty acid elongase complex regulates cell membrane integrity and septin-dependent host infection by the rice blast fungus.Molecular plant pathology · 2024Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The already differentiated organs in plants have a remarkable capacity to regenerate new individuals under culture conditions. Plant in vitro regeneration practically starts with the induction of a pluripotent cell mass, the callus, from detached organs on auxin-rich callus-inducing medium (CIM), which is generally required for subsequent regeneration of new bodies. Recent studies show that CIM-induced callus formation occurs from the pericycle or pericycle-like cells through a root developmental pathway, whereas the signals involved in governing callus-forming capacity of pericycle cells remain unknown. Here we report that very-long-chain fatty acids (VLCFAs) play a critical role in confining the pericycle competence for callus formation and thus the regeneration capacity of Arabidopsis By genetic screening, we identified the callus formation-related 1 (cfr1) mutant, which bypasses the inhibition of callus-forming capacity in roots by solitary-root (slr/iaa14). We show that CFR1 encodes 3-ketoacyl-CoA synthase 1 (KCS1), which catalyzes a rate-limiting step of VLCFA biosynthesis. Our biochemical and genetic analyses demonstrate that VLCFAs restrict the pericycle competence for callus formation, at least in part, by regulating the transcription of Aberrant Lateral Root Formation 4 (ALF4). Moreover, we provide evidence that VLCFAs act as cell layer signals to mediate the pericycle competence for callus formation. Taken together, our results identify VLCFAs or their derivatives as the confining signals for mediating the pericycle competence for callus formation and thus the regeneration capacity of plant organs.
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