ArticleProceedings of the National Academy of Sciences of the United States of America2016
Rational elicitation of cold-sensitive phenotypes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Approaches to Study Proteins Encoded by Essential Genes.Proteins · 2026Review
- LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae.Nucleic acids research · 2024Article
- Genomic estimates of mutation and substitution rates contradict the evolutionary speed hypothesis of the latitudinal diversity gradient.Proceedings. Biological sciences · 2023Article
- A de novo ACTB gene pathogenic variant in identical twins with phenotypic variation for hydrops and jejunal atresia.American journal of medical genetics. Part A · 2022Article
- spn-A/rad51 mutant exhibits enhanced genomic damage, cell death and low temperature sensitivity in somatic tissues.Chromosoma · 2021Article
- Ribosomal RNA Biogenesis and Its Response to Chilling Stress inPlant physiology · 2018Article
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Authors and funding
6 authors.
Funding
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Abstract
Cold-sensitive phenotypes have helped us understand macromolecular assembly and biological phenomena, yet few attempts have been made to understand the basis of cold sensitivity or to elicit it by design. We report a method for rational design of cold-sensitive phenotypes. The method involves generation of partial loss-of-function mutants, at either buried or functional sites, coupled with selective overexpression strategies. The only essential input is amino acid sequence, although available structural information can be used as well. The method has been used to elicit cold-sensitive mutants of a variety of proteins, both monomeric and dimeric, and in multiple organisms, namely Escherichia coli, Saccharomyces cerevisiae, and Drosophila melanogaster This simple, yet effective technique of inducing cold sensitivity eliminates the need for complex mutations and provides a plausible molecular mechanism for eliciting cold-sensitive phenotypes.
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