ArticleNature communications2016
Tum/RacGAP functions as a switch activating the Pav/kinesin-6 motor.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Elevated RACGAP1 Expression Enhances Malignant Potential in Lung Adenocarcinoma and Serves as a Prognostic Factor.Journal of Cancer · 2024Article
- Centralspindlin proteins Pavarotti and Tumbleweed along with WASH regulate nuclear envelope budding.The Journal of cell biology · 2023Article
- Centralspindlin-mediated transport of RhoGEF positions the cleavage plane for cytokinesis.Science signaling · 2023Article
- Article
- The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.PLoS biology · 2022Article
- The kinesin-like protein Pavarotti functions noncanonically to regulate actin dynamics.The Journal of cell biology · 2020Article
- Proximity labeling reveals novel interactomes in liveDevelopment (Cambridge, England) · 2019Article
- A Cyclin A-Myb-MuvB-Aurora B network regulates the choice between mitotic cycles and polyploid endoreplication cycles.PLoS genetics · 2019Article
- Fascetto interacting protein ensures proper cytokinesis and ploidy.Molecular biology of the cell · 2019Article
- Temporal regulation of epithelium formation mediated by FoxA, MKLP1, MgcRacGAP, and PAR-6.Molecular biology of the cell · 2017Article
- The chromosomal basis of meiotic acentrosomal spindle assembly and function in oocytes.Chromosoma · 2017Review
- Polo kinase mediates the phosphorylation and cellular localization of Nuf/FIP3, a Rab11 effector.Molecular biology of the cell · 2017Article
- Article
- Gliding Assay to Analyze Microtubule-based Motor Protein Dynamics.Bio-protocol · 2017Article
- Anaphase B.Biology · 2016Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Centralspindlin is essential for central spindle and cleavage furrow formation. Drosophila centralspindlin consists of a kinesin-6 motor (Pav/kinesin-6) and a GTPase-activating protein (Tum/RacGAP). Centralspindlin localization to the central spindle is mediated by Pav/kinesin-6. While Tum/RacGAP has well-documented scaffolding functions, whether it influences Pav/kinesin-6 function is less well-explored. Here we demonstrate that both Pav/kinesin-6 and the centralspindlin complex (co-expressed Pav/Tum) have strong microtubule bundling activity. Centralspindlin also has robust plus-end-directed motility. In contrast, Pav/kinesin-6 alone cannot move microtubules. However, the addition of Tum/RacGAP or a 65 amino acid Tum/RacGAP fragment to Pav/kinesin-6 restores microtubule motility. Further, ATPase assays reveal that microtubule-stimulated ATPase activity of centralspindlin is seven times higher than that of Pav/kinesin-6. These findings are supported by in vivo studies demonstrating that in Tum/RacGAP-depleted S2 Drosophila cells, Pav/kinesin-6 exhibits severely reduced localization to the central spindle and an abnormal concentration at the centrosomes.
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Registered trials
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