ArticleScientific reports2016
Properties of Two Enterovirus Antibodies that are Utilized in Diabetes Research.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Coxsackie B1 virus-like particle vaccine modified to exclude a highly conserved immunoreactive region from the capsid induces potent neutralizing antibodies and protects against infection in mice.Journal of biomedical science · 2025Article
- Detection of enterovirus RNA in pancreas and lymphoid tissues of organ donors with type 1 diabetes.Diabetologia · 2025Article
- Detection of enterovirus RNA in pancreas and lymphoid tissues of organ donors with type 1 diabetes.medRxiv : the preprint server for health sciences · 2024Article
- Activation of protein kinase receptor (PKR) plays a pro-viral role in mammarenavirus-infected cells.Journal of virology · 2024Article
- Live enteroviruses, but not other viruses, detected in human pancreas at the onset of type 1 diabetes in the DiViD study.Diabetologia · 2022Article
- High Prevalence of Common Human Viruses in Thyroid Tissue.Frontiers in endocrinology · 2022Article
- Immune Transcriptome of Cells Infected with Enterovirus Strains Obtained from Cases of Autoimmune Thyroid Disease.Microorganisms · 2021Article
- The diabetes pandemic and associated infections: suggestions for clinical microbiology.Reviews in medical microbiology : a journal of the Pathological Society of Great Britain and Ireland · 2019Article
- Enterovirus infection and type 1 diabetes: unraveling the crime scene.Clinical and experimental immunology · 2019Review
- A novel rat CVB1-VP1 monoclonal antibody 3A6 detects a broad range of enteroviruses.Scientific reports · 2018Article
- Antibody fingerprints in lyme disease deciphered with high density peptide arrays.Engineering in life sciences · 2017Article
- Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human enteroviruses (EVs) comprise >100 different types. Research suggests a non-chance association between EV infections and type 1 diabetes. Immunohistochemical studies with the anti-EV antibody 5D-8.1 have shown that the EV capsid antigen is present in pancreatic islet cells of diabetic subjects. When it was noticed that 5D-8.1 may cross-react with human proteins, doubt was casted on the significance of the above histopathologic findings. To address this issue, properties of EV antibodies 5D-8.1 and 9D5 have been investigated using peptide microarrays, peptide substitution scanning, immunofluorescence of EV-infected cells, EV neutralization assays, bioinformatics analysis. Evidence indicates that the two antibodies bind to distinct non-neutralizing linear epitopes in VP1 and are specific for a vast spectrum of EV types (not for other human viruses). However, their epitopes may align with a few human proteins at low expected values. When tested by immunofluorescence, high concentrations of 5D-8.1 yelded faint cytoplasmic staining in uninfected cells. At reduced concentrations, both antibodies produced dotted staining only in the cytoplasm of infected cells and recognized both acute and persistent EV infection. Thus, the two monoclonals represent distinct and independent probes for hunting EVs in tissues of patients with diabetes or other endocrine conditions.
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