ArticleScientific reports2016
Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
141 citing papers in PubMed, 1 synthesis or guideline pooled it, 408 citations in OpenAlex.
- Pooled it
- Revolutionizing vision using corneal 3D printing.Trends in biotechnology · 2026Review
- 3D Bioprinted Alginate-Gelatin-Collagen Based Hydrogels Laden With Fibroblast and Keratinocyte Cells Show Potential for Regeneration of Injured Skin.Macromolecular bioscience · 2026Article
- Flexible, Degradable, and Flame-Retardant CaAlg/CNT/PPy Composite Fiber Electrodes for High-Performance Fiber-Shaped Supercapacitors.ACS omega · 2026Article
- High-Resolution 3D Bioprinted Hydrogel Scaffolds Enable Sustained Intraperitoneal Cell Delivery.Molecules (Basel, Switzerland) · 2026Article
- On-Demand Chemically Degradable Hydrogels for Biological Applications.Chembiochem : a European journal of chemical biology · 2026Review
- Influence of the Intervertebral Disc Microenvironment on Matrix Synthesis and Metabolism in Goat Nucleus Pulposus Cells.JOR spine · 2026Article
- Corneal Epithelial Tissue Engineering Strategy Based on Cell Viability Optimization: A Review and Prospects.Bioengineering (Basel, Switzerland) · 2025Review
- Transformative bioprinting: 4D printing and its role in the evolution of engineering and personalized medicine.Discover nano · 2025Review
- Effect of 3D Printing Parameters on the Transparency of Medical Hydrogels for Corneal Stroma Fabrication.Gels (Basel, Switzerland) · 2025Article
- Bioprintable and injectable TiO-loaded hydrogels for photothermal therapy of glioma.Materials today. Bio · 2025Article
- Advances in 3D Bioprinting for Corneal Regeneration.Gels (Basel, Switzerland) · 2025Review
- NIR Phosphorescent Oxygen Sensors in Natural Hydrogel Matrices.bioRxiv : the preprint server for biology · 2025Article
- Fabrication of a 3D Corneal Model Using Collagen Bioink and Human Corneal Stromal Cells.Journal of functional biomaterials · 2025Article
- Encapsulation of Lactoferrin in Calcium-Alginate Microparticles and Its Release Therefrom Under Neutral and Mild Acidic Conditions: Synthesis, Characterization and Mathematical Modeling.Gels (Basel, Switzerland) · 2025Article
- 3D Bioprinting of Cellular Therapeutic Systems in Ophthalmology: from Bioengineered Tissue to Personalized Drug Delivery.Current ophthalmology reports · 2025Review
- Biodegradable hydrogels and microbial consortia as a treatment for soil dysbiosis.Frontiers in microbiology · 2025Review
- Design and evaluation of ionically crosslinked multifunctional ELP-SA composite hydrogels for 3D cell culture.Regenerative biomaterials · 2025Article
- Innovative Ink-Based 3D Hydrogel Bioprinted Formulations for Tissue Engineering Applications.Gels (Basel, Switzerland) · 2024Article
- Advancements in Polymer Biomaterials as Scaffolds for Corneal Endothelium Tissue Engineering.Polymers · 2024Review
81 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alginate hydrogel is a popular biologically inert material that is widely used in 3D bioprinting, especially in extrusion-based printing. However, the printed cells in this hydrogel could not degrade the surrounding alginate gel matrix, causing them to remain in a poorly proliferating and non-differentiating state. Here, we report a novel study of the 3D printing of human corneal epithelial cells (HCECs)/collagen/gelatin/alginate hydrogel incubated with a medium containing sodium citrate to obtain degradation-controllable cell-laden tissue constructs. The 3D-printed hydrogel network with interconnected channels and a macroporous structure was stable and achieved high cell viability (over 90%). By altering the mole ratio of sodium citrate/sodium alginate, the degradation time of the bioprinting constructs can be controlled. Cell proliferation and specific marker protein expression results also revealed that with the help of sodium citrate degradation, the printed HCECs showed a higher proliferation rate and greater cytokeratin 3(CK3) expression, indicating that this newly developed method may help to improve the alginate bioink system for the application of 3D bioprinting in tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.