ArticleRNA (New York, N.Y.)2016
Proofreading neutralizes potential error hotspots in genetic code translation by transfer RNAs.
Article in RNA (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- RNAViruses · 2026Review
- Article
- Geometric alignment of aminoacyl-tRNA relative to catalytic centers of the ribosome underpins accurate mRNA decoding.Nature communications · 2023Article
- Bridging ribosomal synthesis to cell growth through the lens of kinetics.Biophysical journal · 2023Article
- N 6-Methyladenosines in mRNAs reduce the accuracy of codon reading by transfer RNAs and peptide release factors.Nucleic acids research · 2021Article
- Elongation factor-Tu can repetitively engage aminoacyl-tRNA within the ribosome during the proofreading stage of tRNA selection.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A single H/ACA small nucleolar RNA mediates tumor suppression downstream of oncogenic RAS.eLife · 2019Article
- The multiple flavors of GoU pairs in RNA.Journal of molecular recognition : JMR · 2019Review
- Article
- Broad range of missense error frequencies in cellular proteins.Nucleic acids research · 2019Article
- tRNA tracking for direct measurements of protein synthesis kinetics in live cells.Nature chemical biology · 2018Article
- A Stem-Loop Structure inJournal of virology · 2018Article
- Accuracy of genetic code translation and its orthogonal corruption by aminoglycosides and Mg2+ ions.Nucleic acids research · 2018Article
- Translational fidelity and mistranslation in the cellular response to stress.Nature microbiology · 2017Review
- Dynamic basis of fidelity and speed in translation: Coordinated multistep mechanisms of elongation and termination.Protein science : a publication of the Protein Society · 2017Review
- Article
- Function and origin of mistranslation in distinct cellular contexts.Critical reviews in biochemistry and molecular biology · 2017Review
- A recent intermezzo at the Ribosome Club.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Ribosome dynamics during decoding.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Two proofreading steps amplify the accuracy of genetic code translation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ribosome uses initial and proofreading selection of aminoacyl-tRNAs for accurate protein synthesis. Anomalously high initial misreading in vitro of near-cognate codons by tRNA(His) and tRNA(Glu) suggested potential error hotspots in protein synthesis, but in vivo data suggested their partial neutralization. To clarify the role of proofreading in this error reduction, we varied the Mg(2+) ion concentration to calibrate the total accuracy of our cell-free system to that in the living Escherichia coli cell. We found the total accuracy of tRNA selection in our system to vary by five orders of magnitude depending on tRNA identity, type of mismatch, and mismatched codon position. Proofreading and initial selection were positively correlated at high, but uncorrelated at low initial selection, suggesting hyperactivated proofreading as a means to neutralize potentially disastrous initial selection errors.
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