Evidence map›Paper›PMID 27090082›Full record

ArticleParasites & vectors2016

In vitro permissiveness of bovine neutrophils and monocyte derived macrophages to Leishmania donovani of Ethiopian isolate.

Geremew Tasew, Endalamaw Gadisa, Adugna Abera, Aboma Zewude, Menberework Chanyalew, Abraham Aseffa, Markos Abebe, Uwe Ritter, Ger van Zandbergen, Tamás Laskay and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Geremew TasewEthiopia Public Health Institute, Leishmaniasis Research Laboratory, P.O. Box 1242, Addis Ababa, Ethiopia.
Endalamaw GadisaArmauer Hansen Research Institute, Leishmaniasis Research Laboratory, P.O. Box 1005, Addis Ababa, Ethiopia.
Adugna AberaArmauer Hansen Research Institute, Leishmaniasis Research Laboratory, P.O. Box 1005, Addis Ababa, Ethiopia.
Aboma ZewudeArmauer Hansen Research Institute, Leishmaniasis Research Laboratory, P.O. Box 1005, Addis Ababa, Ethiopia.
Menberework ChanyalewArmauer Hansen Research Institute, Leishmaniasis Research Laboratory, P.O. Box 1005, Addis Ababa, Ethiopia.
Abraham AseffaArmauer Hansen Research Institute, Leishmaniasis Research Laboratory, P.O. Box 1005, Addis Ababa, Ethiopia.
Markos AbebeArmauer Hansen Research Institute, Leishmaniasis Research Laboratory, P.O. Box 1005, Addis Ababa, Ethiopia.
Uwe RitterDepartment of Immunology, University of Regensburg, Franz-Josef-Strauß-Allee 11, D-93042, Regensburg, Germany.
Ger van ZandbergenPaul-Ehrlich-Institute, Federal Institute for Vaccines and Biomedicines, Langen, Germany.
Tamás LaskayInstitute for Medical Microbiology and Hygiene, University of Lübeck, Ratzeburger Allee 160, D-23560, Lübeck, Germany.
Ketema TafessDepartment of Medical Laboratory Science, College of Health Sciences, Arsi University, P.O. Box 193, Asella, Ethiopia. ttafess@gmail.com.
Armauer Hansen Research Institute · ETArsi UniversityPaul Ehrlich Institut · DEUniversity of Lübeck · DEUniversity of Regensburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEpidemiological studies in Ethiopia have documented that the risk of visceral leishmaniasis (VL, Kala-azar) is higher among people living with domestic animals. The recent report on isolation of Leishmania donovani complex DNA and the detected high prevalence of anti-leishmanial antibodies in the blood of domestic animals further strengthen the potential role of domestic animals in the epidemiology of VL in Ethiopia. In mammalian hosts polymorphonuclear cells (PMN) and macrophages are the key immune cells influencing susceptibility or control of Leishmania infection. Thus to substantiate the possible role of cattle in VL transmission we investigate the permissiveness of bovine PMN and monocyte derived macrophages (MDM) for Leishmania (L.) donovani infection.

methodsWhole blood was collected from pure Zebu (Boss indicus) and their cross with Holstein Friesian cattle. L. donovani (MHOM/ET/67/HU3) wild and episomal green fluorescent protein (eGFP) labelled stationary stage promastigotes were co-incubated with whole blood and MDM to determine infection of these cells. Engulfment of promastigotes by the cells and their transformation to amastigote forms in MDM was studied with direct microscopy. Microscopy and flow cytometry were used to measure the infection rate while PCR-RLFP was used to confirm the infecting parasite.

resultsL. donovani infected bovine whole blood PMN in the presence of plasma factors and all cellular elements. Morphological examinations of stained cytospin smears revealed that PMN engulfed promastigotes. Similarly, we were able to show that bovine MDM can be infected by L. donovani, which transformed to amastigote forms in the cells.

conclusionsThe in vitro infection of bovine PMN and MDM by L. donovani further strengthens the possibility that cattle might serve as source of L. donovani infection for humans.

Indexed as

AnimalsCattleCattle DiseasesEthiopiaLeishmania donovaniLeishmaniasis, VisceralMacrophagesNeutrophilsBovine PMNHolstein FriesianMDMReservoir hostZebu

Identifiers

PMID27090082
PMCPMC4836163
OpenAlexW2336221371

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.