Evidence map›Paper›PMID 27090004›Full record

ReviewMethods (San Diego, Calif.)2016

Protein-protein interaction analysis for functional characterization of helicases.

Boris L Zybailov, Alicia K Byrd, Galina V Glazko, Yasir Rahmatallah, Kevin D Raney

Open access · greenAbstract readReview
In one paragraph

Review in Methods (San Diego, Calif.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Trial
  2. Role of DEAD/DEAH-box helicases in immunity, infection and cancers.Cell communication and signaling : CCS · 2025
    Review
  3. Review
  4. Proteomics-inspired precision medicine for treating and understanding multiple myeloma.Expert review of precision medicine and drug development · 2020
    Article
  5. Article
  6. Special Methods collection on DNA helicases.Methods (San Diego, Calif.) · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Boris L ZybailovDepartment of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, United States.
Alicia K ByrdDepartment of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, United States.
Galina V GlazkoDepartment of Medical Informatics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, United States.
Yasir RahmatallahDepartment of Medical Informatics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, United States.
Kevin D RaneyDepartment of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, United States. Electronic address: RaneyKevinD@uams.edu.
University of Arkansas for Medical Sciences · US

Funding

DNA Helicases: Mechanisms and FunctionsR01GM098922 · NIGMS · UNIV OF ARKANSAS FOR MED SCIS · PI RANEY, KEVIN DOUGLAS · 2011 to 2017
$1.7M
G-quadruplex DNA as a chemical signaling agentR01GM117439 · NIGMS · UNIV OF ARKANSAS FOR MED SCIS · PI RANEY, KEVIN DOUGLAS · 2015 to 2016
$589k
NIGMS NIH HHS R01 GM098922NIGMS NIH HHS R01 GM117439
6 · The paper itself

Abstract

Helicases are enzymes involved in nucleic acid metabolism, playing major roles in replication, transcription, and repair. Defining helicases oligomerization state and transient and persistent protein interactions is essential for understanding of their function. In this article we review current methods for the protein-protein interaction analysis, and discuss examples of its application to the study of helicases: Pif1 and DDX3. Proteomics methods are our main focus - affinity pull-downs and chemical cross-linking followed by mass spectrometry. We review advantages and limitations of these methods and provide general guidelines for their implementation in the functional analysis of helicases.

Indexed as

DNA HelicasesDNA RepairDNA ReplicationProtein Interaction MappingProtein Interaction MapsDNA HelicasesBiological networksChemical cross-linkingHelicaseMass spectrometryProtein oligomerizationProtein-protein interactions

Identifiers

PMID27090004
PMCPMC5035562
OpenAlexW2337179210

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.