ArticleScientific reports2016
Detection and characterization of two co-infection variant strains of avian orthoreovirus (ARV) in young layer chickens using next-generation sequencing (NGS).
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Pooled it
- Characterization of a Goose-Origin Avian Orthoreovirus with Interferon Suppression Activity.Viruses · 2026Article
- Co-detection and genomic characterization of avian rotavirus A, avian orthoreovirus, and chicken megrivirus-C using nontargeted metagenomic surveillance in Indian broiler chickens.Frontiers in cellular and infection microbiology · 2026Article
- Orthoreovirus avis in asymptomatic broiler chickens.Tropical animal health and production · 2025Article
- Phylogenetic Analysis and Pathogenicity of Avian Reoviruses Isolated from Viral Arthritis Cases in China 2010-2024.Veterinary sciences · 2025Article
- Choosing the most suitable NGS technology to combine with a standardized viral enrichment protocol for obtaining complete avian orthoreovirus genomes from metagenomic samples.Frontiers in bioinformatics · 2025Article
- Characterization and pathogenicity of a novel avian orthoreovirus in China.Frontiers in microbiology · 2024Article
- Next-Generation Sequencing for the Detection of Microbial Agents in Avian Clinical Samples.Veterinary sciences · 2023Review
- Whole Genomic Constellation of Avian Reovirus Strains Isolated from Broilers with Arthritis in North Carolina, USA.Viruses · 2023Article
- Evidence of the circulation of avian metapneumovirus in domestic backyard chickens in Eastern Saudi Arabia in 2019.Veterinary world · 2023Article
- Isolation and Pathogenicity Analysis of a Novel Orthoreovirus Caused the Outbreak of Duck Viral Arthritis in China.Transboundary and emerging diseases · 2023Article
- Isolation and Molecular Characteristics of a Novel Recombinant Avian Orthoreovirus From Chickens in China.Frontiers in veterinary science · 2021Article
- Sequencing and phylogenetic analysis of partial S1 genes of avian orthoreovirus isolates in Shandong province during 2015-2017.Poultry science · 2020Article
- The dynamics of molecular evolution of emerging avian reoviruses through accumulation of point mutations and genetic re-assortment.Virus evolution · 2020Article
- Establishment of a Real-Time Recombinase Polymerase Amplification Assay for the Detection of Avian Reovirus.Frontiers in veterinary science · 2020Article
- Molecular characterization of two novel reoviruses isolated from Muscovy ducklings in Guangdong, China.BMC veterinary research · 2019Article
- Article
- Molecular and Antigenic Characterization of Piscine orthoreovirus (PRV) from Rainbow Trout (Oncorhynchus mykiss).Viruses · 2018Article
- Genetic and pathogenic characterisation of 11 avian reovirus isolates from northern China suggests continued evolution of virulence.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using next-generation sequencing (NGS) for full genomic characterization studies of the newly emerging avian orthoreovirus (ARV) field strains isolated in Pennsylvania poultry, we identified two co-infection ARV variant strains from one ARV isolate obtained from ARV-affected young layer chickens. The de novo assembly of the ARV reads generated 19 contigs of two different ARV variant strains according to 10 genome segments of each ARV strain. The two variants had the same M2 segment. The complete genomes of each of the two variant strains were 23,493 bp in length, and 10 dsRNA segments ranged from 1192 bp (S4) to 3958 bp (L1), encoding 12 viral proteins. Sequence comparison of nucleotide (nt) and amino acid (aa) sequences of all 10 genome segments revealed 58.1-100% and 51.4-100% aa identity between the two variant strains, and 54.3-89.4% and 49.5-98.1% aa identity between the two variants and classic vaccine strains. Phylogenetic analysis revealed a moderate to significant nt sequence divergence between the two variant and ARV reference strains. These findings have demonstrated the first naturally occurring co-infection of two ARV variants in commercial young layer chickens, providing scientific evidence that multiple ARV strains can be simultaneously present in one host species of chickens.
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