ArticleJournal of biophotonics2017
Label-free in vivo cellular-level detection and imaging of apoptosis.
Article in Journal of biophotonics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Detecting drug-induced nephrotoxicity using simultaneous label-free autofluorescence multiharmonic microscopy.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- Real-time, high-resolution metabolic characterization of live bacteria using label-free optical metabolic imaging.NPJ biofilms and microbiomes · 2026Article
- Accelerating biopharmaceutical cell line selection with label-free multimodal nonlinear optical microscopy and machine learning.Communications biology · 2025Article
- Label-free fluorescence lifetime imaging for the assessment of cell viability in living tumor fragments.Journal of biomedical optics · 2024Article
- Label-free biomedical optical imaging.Nature photonics · 2023Article
- In vivo label-free optical signatures of chemotherapy response in human pancreatic ductal adenocarcinoma patient-derived xenografts.Communications biology · 2023Article
- Label-Free Metabolic Imaging In Vivo by Two-Photon Fluorescence Lifetime Endomicroscopy.ACS photonics · 2022Article
- LANCE: a Label-Free Live Apoptotic and Necrotic Cell Explorer Using Convolutional Neural Network Image Analysis.Analytical chemistry · 2022Article
- Data-Driven Identification of Biomarkers for In Situ Monitoring of Drug Treatment in Bladder Cancer Organoids.International journal of molecular sciences · 2022Article
- Apoptotic Bodies in the Pancreatic Tumor Cell Culture Media Enable Label-Free Drug Sensitivity Assessment by Impedance Cytometry.Advanced biology · 2021Article
- High-speed label-free two-photon fluorescence microscopy of metabolic transients during neuronal activity.Applied physics letters · 2021Article
- Label-Free Multiphoton Microscopy for the Detection and Monitoring of Calcific Aortic Valve Disease.Frontiers in cardiovascular medicine · 2021Review
- Label-Free Multimodal Multiphoton Intravital Imaging.Advances in experimental medicine and biology · 2021Article
- Tissue Imaging and Quantification Relying on Endogenous Contrast.Advances in experimental medicine and biology · 2021Review
- Optical changes in THP-1 macrophage metabolism in response to pro- and anti-inflammatory stimuli reported by label-free two-photon imaging.Journal of biomedical optics · 2020Article
- Tracking metabolic dynamics of apoptosis with high-speed two-photon fluorescence lifetime imaging microscopy.Biomedical optics express · 2019Article
- Digital staining through the application of deep neural networks to multi-modal multi-photon microscopy.Biomedical optics express · 2019Article
- Fluorescence lifetime shifts of NAD(P)H during apoptosis measured by time-resolved flow cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy.Optica · 2018Article
- Investigating the healing mechanisms of an angiogenesis-promoting topical treatment for diabetic wounds using multimodal microscopy.Journal of biophotonics · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Cell death plays a critical role in health and homeostasis as well as in the pathogenesis and treatment of a broad spectrum of diseases and can be broadly divided into two main categories: apoptosis, or programmed cell death, and necrosis, or acute cell death. While these processes have been characterized extensively in vitro, label-free detection of apoptosis and necrosis at the cellular level in vivo has yet to be shown. In this study, for the first time, fluorescence lifetime imaging microscopy (FLIM) of intracellular reduced nicotinamide adenine dinucleotide (NADH) was utilized to assess the metabolic response of in vivo mouse epidermal keratinocytes following induction of apoptosis and necrosis. Results show significantly elevated levels of both the mean lifetime of NADH and the intracellular ratio of protein bound-to-free NADH in the apoptotic compared to the necrotic tissue. In addition, the longitudinal profiles of these two cell death processes show remarkable differences. By identifying and extracting these temporal metabolic signatures, apoptosis in single cells can be studied in native tissue environments within the living organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.