ArticleProteomics2016
Application of wide selected-ion monitoring data-independent acquisition to identify tomato fruit proteins regulated by the CUTIN DEFICIENT2 transcription factor.
Article in Proteomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Data-independent acquisition-based quantitative proteomics in plants.Frontiers in plant science · 2026Review
- Isomerized and Racemized Aspartyl and Deamidated Asparagine Residues Identified in ɣS-Crystallin.Chembiochem : a European journal of chemical biology · 2025Article
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- Dear-DIAResearch (Washington, D.C.) · 2023Article
- RbgA ensures the correct timing in the maturation of the 50S subunits functional sites.Nucleic acids research · 2022Article
- Recent advances in proteomics and metabolomics in plants.Molecular horticulture · 2022Review
- Proteomics Data Analysis for the Identification of Proteins and Derived Proteotypic Peptides of Potential Use as Putative Drought Tolerance Markers forInternational journal of molecular sciences · 2021Article
- Unraveling Cuticle Formation, Structure, and Properties by Using Tomato Genetic Diversity.Frontiers in plant science · 2021Review
- Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development.Frontiers in genetics · 2021Article
- Changes in the microsomal proteome of tomato fruit during ripening.Scientific reports · 2019Article
- Large Scale Proteomic Data and Network-Based Systems Biology Approaches to Explore the Plant World.Proteomes · 2018Article
- Article
- Comparative Analyses of Data Independent Acquisition Mass Spectrometric Approaches: DIA, WiSIM-DIA, and Untargeted DIA.Proteomics · 2018Article
- Early Cold-Induced Peroxidases and Aquaporins Are Associated With High Cold Tolerance in Dajiao (Frontiers in plant science · 2018Article
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Authors and funding
9 authors.
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Abstract
We describe here the use of label-free wide selected-ion monitoring data-independent acquisition (WiSIM-DIA) to identify proteins that are involved in the formation of tomato (Solanum lycopersicum) fruit cuticles and that are regulated by the transcription factor CUTIN DEFICIENT2 (CD2). A spectral library consisting of 11 753 unique peptides, corresponding to 2338 tomato protein groups, was used and the DIA analysis was performed at the MS1 level utilizing narrow mass windows for extraction with Skyline 2.6 software. We identified a total of 1140 proteins, 67 of which had expression levels that differed significantly between the cd2 tomato mutant and the wild-type cultivar M82. Differentially expressed proteins including a key protein involved in cutin biosynthesis, were selected for validation by target SRM/MRM and by Western blot analysis. In addition to confirming a role for CD2 in regulating cuticle formation, the results also revealed that CD2 influences pathways associated with cell wall biology, anthocyanin biosynthesis, plant development, and responses to stress, which complements findings of earlier RNA-Seq experiments. Our results provide new insights into molecular processes and aspects of fruit biology associated with CD2 function, and demonstrate that the WiSIM-DIA is an effective quantitative approach for global protein identifications.
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