ArticleNature methods2016
siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.
Article in Nature methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- A computational super-resolution framework for multidimensional fluorescence imaging.Science advances · 2026Article
- Emerging trends of fluorescence lifetime imaging microscopy (FLIM): advances, challenges, and prospects.Biophysics reports · 2026Article
- Phasor EO-FLIM: Lifetime imaging with picosecond noise and 500 Hz frame rate.bioRxiv : the preprint server for biology · 2026Article
- Artificial Intelligence in Label-Free Optical Imaging Applications.Methods in molecular biology (Clifton, N.J.) · 2026Article
- High-speed wide-field fluorescence lifetime imaging for intraoperative tumor visualization and in vivo multiplexing.Biomedical optics express · 2025Article
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.eLife · 2025Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.bioRxiv : the preprint server for biology · 2025Article
- In Vivo Neurodynamics Mapping via High-Speed Two-Photon Fluorescence Lifetime Volumetric Projection Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM.Communications biology · 2024Article
- High-throughput fluorescence lifetime imaging flow cytometry.Nature communications · 2024Article
- "Radical" differences between two FLIM microscopes affect interpretation of cell signaling dynamics.iScience · 2024Article
- Fast and slow: Recording neuromodulator dynamics across both transient and chronic time scales.Science advances · 2024Article
- Article
- Wide-field fluorescence lifetime imaging of neuron spiking and subthreshold activity in vivo.Science (New York, N.Y.) · 2023Article
- Fluorescence lifetime tracking and imaging of single moving particles assisted by a low-photon-count analysis algorithm.Biomedical optics express · 2023Article
- Identifying lipid particle sub-types in liveFrontiers in chemistry · 2023Article
- Lifetime-based analysis of binary fluorophores mixtures in the low photon count limit.iScience · 2022Article
- A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium.Nature communications · 2021Article
- Dynamic FRET-FLIM based screening of signal transduction pathways.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.