ArticleMetallomics : integrated biometal science2016
Copper-responsive gene expression in the methanotroph Methylosinus trichosporium OB3b.
Article in Metallomics : integrated biometal science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 63 citations in OpenAlex.
- Membrane properties modulate methane oxidation by particulate methane monooxygenase.The Journal of biological chemistry · 2026Article
- A redox- and proton-coupled inner membrane transporter mediates copper import to the bacterial cytoplasm.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Navigating the copper highway: bacterial strategies for cytoplasmic import.Journal of inorganic biochemistry · 2026Review
- Determination of the transcription unit landscape and associated regulatory elements inMicrobiology spectrum · 2025Article
- The Escherichia coli AZY operon links copper uptake to antibiotic resistance.Communications biology · 2025Article
- Methanobactins: Structures, Biosynthesis, and Microbial Diversity.Annual review of microbiology · 2024Review
- Weathered granites and soils harbour microbes with lanthanide-dependent methylotrophic enzymes.BMC biology · 2024Article
- Direct Methane Oxidation by Copper- and Iron-Dependent Methane Monooxygenases.Chemical reviews · 2024Review
- Distribution of methanogenic and methanotrophic consortia at soil-water interfaces in rice paddies across climate zones.iScience · 2023Article
- Mechanism of Action of Ribosomally Synthesized and Post-Translationally Modified Peptides.Chemical reviews · 2022Review
- Structure and activity of particulate methane monooxygenase arrays in methanotrophs.Nature communications · 2022Article
- ThemBio · 2022Article
- Two TonB-Dependent Transporters in Methylosinus trichosporium OB3b Are Responsible for Uptake of Different Forms of Methanobactin and Are Involved in the Canonical "Copper Switch".Applied and environmental microbiology · 2022Article
- The copper-linked Escherichia coli AZY operon: Structure, metal binding, and a possible physiological role in copper delivery.The Journal of biological chemistry · 2022Article
- Antimicrobial Activity of Metals and Metalloids.Annual review of microbiology · 2021Review
- Characterization of a Copper-Chelating Natural Product from the MethanotrophBiochemistry · 2021Article
- Copper binding by a unique family of metalloproteins is dependent on kynurenine formation.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Biochemistry of aerobic biological methane oxidation.Chemical Society reviews · 2021Review
- The model structure of the copper-dependent ammonia monooxygenase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2020Article
- Cu Homeostasis in Bacteria: The Ins and Outs.Membranes · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Methanotrophic bacteria convert methane to methanol using methane monooxygenase (MMO) enzymes. In many strains, either an iron-containing soluble (sMMO) or a copper-containing particulate (pMMO) enzyme can be produced depending on copper availability; the mechanism of this copper switch has not been elucidated. A key player in methanotroph copper homeostasis is methanobactin (Mbn), a ribosomally produced, post-translationally modified natural product with a high affinity for copper. The Mbn precursor peptide is encoded within an operon that contains a range of putative transporters, regulators, and biosynthetic proteins, but the involvement of these genes in Mbn-related processes remains unclear. Extensive time-dependent qRT-PCR studies of Methylosinus trichosporium OB3b and the constitutive sMMO-producing mutant M. trichosporium OB3b PP358 show that the Mbn operon is indeed copper-regulated, providing experimental support for its bioinformatics-based identification. Moreover, the Mbn operon is co-regulated with the sMMO operon and reciprocally regulated with the pMMO operon. Within the Mbn and sMMO operons, a subset of regulatory genes exhibits a distinct and shared pattern of expression, consistent with their proposed functions as internal regulators. In addition, genome sequencing of the M. trichosporium OB3b PP358 mutant provides new evidence for the involvement of genes adjacent to the pMMO operon in methanotroph copper homeostasis.
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Registered trials
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