ArticleCurrent microbiology2016
Searching for Beta-Haemolysin hlb Gene in Staphylococcus pseudintermedius with Species-Specific Primers.
Article in Current microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Whole Genome Sequence Analysis of Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- Review
- Antimicrobial Activity of the Iron-Chelator, DIBI, against Multidrug-Resistant Canine Methicillin-SusceptiblePathogens (Basel, Switzerland) · 2022Article
- Comparative Genomic Reveals Clonal Heterogeneity in PersistentFrontiers in cellular and infection microbiology · 2022Article
- Antimicrobial Resistance of Coagulase-PositiveAntibiotics (Basel, Switzerland) · 2020Article
- Antimicrobial Activity of Some Essential Oils against Methicillin-Susceptible and Methicillin-ResistantAnimals : an open access journal from MDPI · 2020Article
- Staphylococcus pseudintermedius: an undocumented, emerging pathogen in humans.GMS hygiene and infection control · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The paper presents an analysis of 51 Staphylococcus pseudintermedius clinically isolated strains from humans and from animals. Staphylococcus pseudintermedius strains' ability to produce β-haemolysin was evaluated with phenotypic methods (hot-cold effect, reverse CAMP test). In order to determine the hlb gene presence (coding for β-haemolysin) in a genomic DNA, PCR reactions were conducted with two different pairs of primers: one described in the literature for Staphylococcus aureus and recommended for analysing SIG group staphylococci and newly designed one in CLC Main Workbench software. Only reactions with newly designed primers resulted in product amplification, the presence of which was fully compatible with the results of phenotypic β-haemolysin test. Negative results for S. aureus and S. intermedius reference ATCC strains suggest that after further analysis the fragment of hlb gene amplified with primers described in this study might be included in the process of S. pseudintermedius strains identification.
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Registered trials
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