ArticleArchives of dermatological research2016
Functional testing of topical skin formulations using an optimised ex vivo skin organ culture model.
Article in Archives of dermatological research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Assessment of Transdermal Delivery of Topical Compounds in Skin Scarring Using a Novel Combined Approach of Raman Spectroscopy and High-Performance Liquid Chromatography.Advances in wound care · 2021Trial
- Protective Effects of Antioxidant Mix Pre-Treatment Against Visible Light-Induced Damage in Dark Skin Phototype.Antioxidants (Basel, Switzerland) · 2026Article
- Self-assembly of short biopeptides onto skin tissue components studied using QCM-D.International journal of cosmetic science · 2025Article
- Green Tea Catechins and Skin Health.Antioxidants (Basel, Switzerland) · 2024Review
- A human ex vivo skin model breaking boundaries.Scientific reports · 2024Article
- Natural products for the treatment of hypertrophic scars: Preclinical and clinical studies.Heliyon · 2024Review
- Recent insights into catechins-rich Assam tea extract for photoaging and senescent ageing.Scientific reports · 2024Article
- Recreating Human Skin In Vitro: Should the Microbiota Be Taken into Account?International journal of molecular sciences · 2024Review
- A Novel Epidermis Model Using Primary Hidradenitis Suppurativa Keratinocytes.Journal of tissue engineering and regenerative medicine · 2024Article
- Nanotechnology-based alternatives for the topical delivery of immunosuppressive agents in psoriasis.International journal of pharmaceutics · 2023Review
- Controlling Inflammation Pre-Emptively or at the Time of Cutaneous Injury Optimises Outcome of Skin Scarring.Frontiers in immunology · 2022Review
- Article
- Mast Cells in Skin Scarring: A Review of Animal and Human Research.Frontiers in immunology · 2020Article
- Blueberry Extracts as a Novel Approach to Prevent Ozone-Induced Cutaneous Inflammasome Activation.Oxidative medicine and cellular longevity · 2020Article
- Review
- Ex vivo culture of keratinocytes on papillary and reticular dermal layers remodels skin explants differently: towards improved wound care.Archives of dermatological research · 2019Article
- Autograft microskin combined with adipose-derived stem cell enhances wound healing in a full-thickness skin defect mouse model.Stem cell research & therapy · 2019Article
- Luteolin-7-glucoside Promotes Human Epidermal Stem Cell Proliferation by UpregulatingStem cells international · 2019Article
- A Pilot Study to Evaluate the Effects of Topically Applied Cosmetic Creams on Epidermal Responses.Skin pharmacology and physiology · 2018Article
- In vitro psoriasis models with focus on reconstructed skin models as promising tools in psoriasis research.Experimental biology and medicine (Maywood, N.J.) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A number of equivalent-skin models are available for investigation of the ex vivo effect of topical application of drugs and cosmaceuticals onto skin, however many have their drawbacks. With the March 2013 ban on animal models for cosmetic testing of products or ingredients for sale in the EU, their utility for testing toxicity and effect on skin becomes more relevant. The aim of this study was to demonstrate proof of principle that altered expression of key gene and protein markers could be quantified in an optimised whole tissue biopsy culture model. Topical formulations containing green tea catechins (GTC) were investigated in a skin biopsy culture model (n = 11). Punch biopsies were harvested at 3, 7 and 10 days, and analysed using qRT-PCR, histology and HPLC to determine gene and protein expression, and transdermal delivery of compounds of interest. Reduced gene expression of α-SMA, fibronectin, mast cell tryptase, mast cell chymase, TGF-β1, CTGF and PAI-1 was observed after 7 and 10 days compared with treated controls (p < 0.05). Histological analysis indicated a reduction in mast cell tryptase and chymase positive cell numbers in treated biopsies compared with untreated controls at day 7 and day 10 (p < 0.05). Determination of transdermal uptake indicated that GTCs were detected in the biopsies. This model could be adapted to study a range of different topical formulations in both normal and diseased skin, negating the requirement for animal models in this context, prior to study in a clinical trial environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.