ArticleNucleic acids research2016
Two sets of RNAi components are required for heterochromatin formation in trans triggered by truncated transgenes.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Exogenous dsRNA made accessible to Dicer by two eukaryotic RNA-dependent RNA polymerases in Paramecium tetraurelia.Communications biology · 2026Article
- Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium.Cell reports · 2023Article
- Identification of novel, functional, long noncoding RNAs involved in programmed, large-scale genome rearrangements.RNA (New York, N.Y.) · 2022Article
- Broad domains of histone marks in the highly compactGenome research · 2022Article
- Article
- Feeding exogenous dsRNA interferes with endogenous sRNA accumulation in Paramecium.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Article
- Programmed Chromosome Deletion in the CiliateG3 (Bethesda, Md.) · 2019Article
- Exogenous RNAi mechanisms contribute to transcriptome adaptation by phased siRNA clusters in Paramecium.Nucleic acids research · 2019Article
- Article
- Environmental Temperature Controls Accumulation of Transacting siRNAs Involved in Heterochromatin Formation.Genes · 2018Article
- Two Sets of Piwi Proteins Are Involved in Distinct sRNA Pathways Leading to Elimination of Germline-Specific DNA.Cell reports · 2017Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Across kingdoms, RNA interference (RNAi) has been shown to control gene expression at the transcriptional- or the post-transcriptional level. Here, we describe a mechanism which involves both aspects: truncated transgenes, which fail to produce intact mRNA, induce siRNA accumulation and silencing of homologous loci in trans in the ciliate Paramecium We show that silencing is achieved by co-transcriptional silencing, associated with repressive histone marks at the endogenous gene. This is accompanied by secondary siRNA accumulation, strictly limited to the open reading frame of the remote locus. Our data shows that in this mechanism, heterochromatic marks depend on a variety of RNAi components. These include RDR3 and PTIWI14 as well as a second set of components, which are also involved in post-transcriptional silencing: RDR2, PTIWI13, DCR1 and CID2. Our data indicates differential processing of nascent un-spliced and long, spliced transcripts thus suggesting a hitherto-unrecognized functional interaction between post-transcriptional and co-transcriptional RNAi. Both sets of RNAi components are required for efficient trans-acting RNAi at the chromatin level and our data indicates similar mechanisms contributing to genome wide regulation of gene expression by epigenetic mechanisms.
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