ArticleCellular microbiology2016
Palmitoyl transferases have critical roles in the development of mosquito and liver stages of Plasmodium.
Article in Cellular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Protein palmitoylation is involved in regulating mouse sperm motility via the signals of calcium, protein tyrosine phosphorylation and reactive oxygen species.Biological research · 2025Article
- Widespread release of translational repression across Plasmodium's host-to-vector transmission event.PLoS pathogens · 2025Article
- Post-Translational Modifications of Proteins of Malaria Parasites during the Life Cycle.International journal of molecular sciences · 2024Review
- Global profiling of protein S-palmitoylation in the second-generation merozoites of Eimeria tenella.Parasitology research · 2024Article
- Global Release of Translational Repression AcrossbioRxiv : the preprint server for biology · 2024Article
- Plasmodium sporozoite disintegration during skin passage limits malaria parasite transmission.EMBO reports · 2022Article
- Article
- Characterization of PSOP26 as an ookinete surface antigen with improved transmission-blocking activity when fused with PSOP25.Parasites & vectors · 2022Article
- Article
- Identification of Three NovelFrontiers in cellular and infection microbiology · 2021Article
- A protein palmitoylation cascade regulates microtubule cytoskeleton integrity in Plasmodium.The EMBO journal · 2020Article
- The Dynamic Roles of the Inner Membrane Complex in the Multiple Stages of the Malaria Parasite.Frontiers in cellular and infection microbiology · 2020Review
- Deletion of the rodent malaria ortholog for falcipain-1 highlights differences between hepatic and blood stage merozoites.PLoS pathogens · 2017Article
- Dynamic protein S-palmitoylation mediates parasite life cycle progression and diverse mechanisms of virulence.Critical reviews in biochemistry and molecular biology · 2017Review
- Palmitoylation of Plasmodium alveolins promotes cytoskeletal function.Molecular and biochemical parasitology · 2017Article
- Protein S-palmitoylation in cellular differentiation.Biochemical Society transactions · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
As the Plasmodium parasite transitions between mammalian and mosquito host, it has to adjust quickly to new environments. Palmitoylation, a reversible and dynamic lipid post-translational modification, plays a central role in regulating this process and has been implicated with functions for parasite morphology, motility and host cell invasion. While proteins associated with the gliding motility machinery have been described to be palmitoylated, no palmitoyl transferase responsible for regulating gliding motility has previously been identified. Here, we characterize two palmityol transferases with gene tagging and gene deletion approaches. We identify DHHC3, a palmitoyl transferase, as a mediator of ookinete development, with a crucial role for gliding motility in ookinetes and sporozoites, and we co-localize the protein with a marker for the inner membrane complex in the ookinete stage. Ookinetes and sporozoites lacking DHHC3 are impaired in gliding motility and exhibit a strong phenotype in vivo; with ookinetes being significantly less infectious to their mosquito host and sporozoites being non-infectious to mice. Importantly, genetic complementation of the DHHC3-ko parasite completely restored virulence. We generated parasites lacking both DHHC3, as well as the palmitoyl transferase DHHC9, and found an enhanced phenotype for these double knockout parasites, allowing insights into the functional overlap and compensational nature of the large family of PbDHHCs. These findings contribute to our understanding of the organization and mechanism of the gliding motility machinery, which as is becoming increasingly clear, is mediated by palmitoylation.
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