Evidence map›Paper›PMID 27084428›Full record

ArticleTranslational oncology2016

Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.

C Rory Goodwin, Crystal L Woodard, Xin Zhou, Jianbo Pan, Alessandro Olivi, Shuli Xia, Chetan Bettegowda, Daniel M Sciubba, Jonathan Pevsner, Heng Zhu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational oncology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

C Rory GoodwinDepartment of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA. Electronic address: rory@jhmi.edu.
Crystal L WoodardDepartment of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Xin ZhouDepartment of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA.
Jianbo PanDepartment of Ophthalmology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Alessandro OliviDepartment of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Shuli XiaHugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA.
Chetan BettegowdaDepartment of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Daniel M SciubbaDepartment of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Jonathan PevsnerHugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA.
Heng ZhuDepartment of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
John LaterraDepartment of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA.

Funding

Mechanisms of Chemo/Radioresistance in Human GliomasR01NS043987 · NINDS · HUGO W. MOSER RES INST KENNEDY KRIEGER · PI LATERRA, JOHN J, ROSEN, ELIOT M. · 2003 to 2012
$4.9M
Suppression of Glioblastoma Stem Cells by Kruppel-Like Factor 9R01NS076759 · NINDS · HUGO W. MOSER RES INST KENNEDY KRIEGER · PI LATERRA, JOHN J · 2011 to 2015
$1.6M
Neutralizing Anti-HGF mAbs and CNS MalignancyR01CA129192 · NCI · HUGO W. MOSER RES INST KENNEDY KRIEGER · PI LATERRA, JOHN J · 2007 to 2010
$1.2M
NCI NIH HHS R01 CA129192NINDS NIH HHS R01 NS043987NINDS NIH HHS R01 NS076759
6 · The paper itself

Abstract

Protein microarray technology has been successfully used for identifying substrates of purified activated kinases. We used protein microarrays to globally interrogate the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo glioma models and validated results in clinical pathological specimens. Whole cell lysates extracted from tumor samples can be applied to human kinome chip microarrays to profile the global kinase phosphorylation patterns in a high-throughput manner and identify novel substrates inherent to the tumor cell and the interactions with tumor microenvironment. Our findings identify a novel microarray-based method for assessing intracellular signaling events applicable to human oncogenesis and other pathophysiologic states.

Identifiers

PMID27084428
PMCPMC4833893

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.