ArticlePloS one2016
Genome-Wide Identification and Characterization of microRNAs in Developing Grains of Zea mays L.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 65 citations in OpenAlex.
- MicroRNA528 and Its Regulatory Roles in Monocotyledonous Plants.International journal of molecular sciences · 2025Review
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- Noncoding RNAs and their roles in regulating the agronomic traits of crops.Fundamental research · 2023Review
- Transcriptional Regulation of zma-International journal of molecular sciences · 2022Article
- Developing Genetic Engineering Techniques for Control of Seed Size and Yield.International journal of molecular sciences · 2022Review
- Identification and characterization of miRNAs and PHAS loci related to the early development of the embryo and endosperm in Fragaria × ananassa.BMC genomics · 2022Article
- Regulators of Starch Biosynthesis in Cereal Crops.Molecules (Basel, Switzerland) · 2021Review
- Rice miR1432 Fine-Tunes the Balance of Yield and Blast Disease Resistance via Different Modules.Rice (New York, N.Y.) · 2021Article
- MicroRNA Zma-miR528 Versatile Regulation on Target mRNAs during Maize Somatic Embryogenesis.International journal of molecular sciences · 2021Article
- Genomic imprinted genes in reciprocal hybrid endosperm of Brassica napus.BMC plant biology · 2021Article
- Integrated microRNA and transcriptome profiling reveal key miRNA-mRNA interaction pairs associated with seed development in Tartary buckwheat (Fagopyrum tataricum).BMC plant biology · 2021Article
- Genome-wide profiling of miRNAs during seed development reveals their functional relevance in seed size/weight determination in chickpea.Plant direct · 2021Article
- Article
- Multi-Omics Analysis of Small RNA, Transcriptome, and Degradome inInternational journal of molecular sciences · 2020Article
- Epigenomic landscape and epigenetic regulation in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Movement of small RNAs in and between plants and fungi.Molecular plant pathology · 2020Review
- Elucidating the regulatory roles of microRNAs in maize (Zea mays L.) leaf growth response to chilling stress.Planta · 2020Article
- Analysis of the genetic architecture of maize kernel size traits by combined linkage and association mapping.Plant biotechnology journal · 2020Article
- The explant developmental stage profoundly impacts small RNA-mediated regulation at the dedifferentiation step of maize somatic embryogenesis.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development and maturation of maize kernel involves meticulous and fine gene regulation at transcriptional and post-transcriptional levels, and miRNAs play important roles during this process. Although a number of miRNAs have been identified in maize seed, the ones involved in the early development of grains and in different lines of maize have not been well studied. Here, we profiled four small RNA libraries, each constructed from groups of immature grains of Zea mays inbred line Chang 7-2 collected 4-6, 7-9, 12-14, and 18-23 days after pollination (DAP). A total of 40 known (containing 111 unique miRNAs) and 162 novel (containing 196 unique miRNA candidates) miRNA families were identified. For conserved and novel miRNAs with over 100 total reads, 44% had higher accumulation before the 9th DAP, especially miR166 family members. 42% of miRNAs had highest accumulation during 12-14 DAP (which is the transition stage from embryogenesis to nutrient storage). Only 14% of miRNAs had higher expression 18-23 DAP. Prediction of potential targets of all miRNAs showed that 165 miRNA families had 377 target genes. For miR164 and miR166, we showed that the transcriptional levels of their target genes were significantly decreased when co-expressed with their cognate miRNA precursors in vivo. Further analysis shows miR159, miR164, miR166, miR171, miR390, miR399, and miR529 families have putative roles in the embryogenesis of maize grain development by participating in transcriptional regulation and morphogenesis, while miR167 and miR528 families participate in metabolism process and stress response during nutrient storage. Our study is the first to present an integrated dynamic expression pattern of miRNAs during maize kernel formation and maturation.
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Registered trials
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