ArticleJournal of nuclear medicine : official publication, Society of Nuclear Medicine2016
Single-Cell Characterization of 18F-FLT Uptake with Radioluminescence Microscopy.
Article in Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
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- Novel multi-omics analysis revealing metabolic heterogeneity of breast cancer cell and subsequent development of associated prognostic signature.Translational oncology · 2025Article
- High mitochondrial DNA content is a key determinant of stemness, proliferation, cell migration, and cancer metastasis in vivo.Cell death & disease · 2024Article
- Inorganic Nanoparticles as Radiosensitizers for Cancer Treatment.Nanomaterials (Basel, Switzerland) · 2023Review
- Development of a Lensless Radiomicroscope for Cellular-Resolution Radionuclide Imaging.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2023Article
- Direct Cell Radiolabeling forChemical reviews · 2022Review
- Flow radiocytometry using droplet optofluidics.Biosensors & bioelectronics · 2021Article
- Microfluidics-Coupled Radioluminescence Microscopy forAnalytical chemistry · 2021Article
- Single-cell radioluminescence microscopy with two-fold higher sensitivity using dual scintillator configuration.PloS one · 2020Article
- Radioluminescence in biomedicine: physics, applications, and models.Physics in medicine and biology · 2019Review
- Single-Cell Imaging Using Radioluminescence Microscopy Reveals Unexpected Binding Target for [18F]HFB.Molecular imaging and biology · 2018Article
- Development and characterization of a scintillating cell imaging dish for radioluminescence microscopy.The Analyst · 2018Article
- In silico optimization of radioluminescence microscopy.Journal of biophotonics · 2018Article
- Toward a Droplet-Based Single-Cell Radiometric Assay.Analytical chemistry · 2017Article
- Performance evaluation ofMedical physics · 2017Article
- Modular low-light microscope for imaging cellular bioluminescence and radioluminescence.Nature protocols · 2017Article
- Optical Imaging of Ionizing Radiation from Clinical Sources.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2016Review
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2 authors at 1 institution in 1 country.
Funding
Abstract
unlabelledThe radiotracer 3'-deoxy-3'-(18)F-fluorothymidine ((18)F-FLT) is commonly used to measure cell proliferation in vivo. As a marker of cell proliferation, (18)F-FLT is expected to be differentially taken up by arrested and actively dividing cells, but PET measures only aggregate uptake by tumor cells and therefore the single-cell distribution of (18)F-FLT is unknown. We used a novel in vitro radioluminescence microscopy technique to measure the differential distribution of (18)F-FLT radiotracer with single-cell precision.
methodsUsing radioluminescence microscopy, we imaged the absolute uptake of (18)F-FLT in live MDA-MB-231 cells grown under different serum conditions. We then compared (18)F-FLT uptake with a standard measure of cell proliferation, using fluorescence microscopy of 5-ethynyl-2'-deoxyuridine incorporation in fixed cells.
resultsAccording to 5-ethynyl-2'-deoxyuridine staining, few cells (1%) actively cycled under serum deprivation whereas most of them (71%) did under 20% serum. The distribution of (18)F-FLT reflected this dynamic. At 0% serum, uptake of (18)F-FLT was heterogeneous but relatively low. At 20% serum, a subpopulation of (18)F-FLT-avid cells, representing 61% of the total population, emerged. Uptake of (18)F-FLT in this population was 5-fold higher than in the remainder of the cells. Such a dichotomous distribution is not typically observed with other radiotracers, such as (18)F-FDG.
conclusionThese results suggest that increased (18)F-FLT uptake by proliferating cells is due to a greater fraction of (18)F-FLT-avid cells rather than a change in (18)F-FLT uptake by individual cells. This finding is consistent with the fact that (18)F-FLT uptake is mediated by thymidine kinase 1 expression, which is higher in actively dividing cells. Overall, these findings suggest that, within the same patient, changes in (18)F-FLT uptake reflect changes in the number of actively dividing cells, provided other parameters remain the same.
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