ArticleScientific reports2016
Integrative Phosphoproteomics Links IL-23R Signaling with Metabolic Adaptation in Lymphocytes.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Targeting pyruvate kinase M2 (PKM2) reduces T cell pathogenicity in multiple sclerosis.EBioMedicine · 2026Article
- Functions and modulation of PKM2 activity by human papillomavirus E7 oncoprotein (Review).Oncology letters · 2023Review
- Mechanism of PKM2 affecting cancer immunity and metabolism in Tumor Microenvironment.Journal of Cancer · 2021Review
- Control of T Cell Metabolism by Cytokines and Hormones.Frontiers in immunology · 2021Review
- PKM2 promotes Th17 cell differentiation and autoimmune inflammation by fine-tuning STAT3 activation.The Journal of experimental medicine · 2020Article
- IL-12 and IL-23-Close Relatives with Structural Homologies but Distinct Immunological Functions.Cells · 2020Review
- Review
- Blocking inflammation to improve immunotherapy of advanced cancer.Immunology · 2020Review
- IL-23 signaling regulation of pro-inflammatory T-cell migration uncovered by phosphoproteomics.PLoS biology · 2020Article
- Pharmacological Activation of Pyruvate Kinase M2 Inhibits CD4Cell metabolism · 2020Article
- SPRTN protease and checkpoint kinase 1 cross-activation loop safeguards DNA replication.Nature communications · 2019Article
- Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells.Nature communications · 2019Article
- The IL-12 Cytokine and Receptor Family in Graft-vs.-Host Disease.Frontiers in immunology · 2019Review
- Bi-directional signaling by membrane-bound KitL induces proliferation and coordinates thymic endothelial cell and thymocyte expansion.Nature communications · 2018Article
- Inhibiting ex-vivo Th17 responses in Ankylosing Spondylitis by targeting Janus kinases.Scientific reports · 2018Article
- PKM2-dependent metabolic reprogramming in CD4Journal of molecular medicine (Berlin, Germany) · 2018Article
- Article
- Synthetic Deletion of the Interleukin 23 Receptor (IL-23R) Stalk Region Led to Autonomous IL-23R Homodimerization and Activation.Molecular and cellular biology · 2017Article
- Lighting Up T Lymphocyte Signaling with Quantitative Phosphoproteomics.Frontiers in immunology · 2017Review
- PKM2, cancer metabolism, and the road ahead.EMBO reports · 2016Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Interleukin (IL)-23 mediated signal transduction represents a major molecular mechanism underlying the pathology of inflammatory bowel disease, Crohn's disease and ulcerative colitis. In addition, emerging evidence supports the role of IL-23-driven Th17 cells in inflammation. Components of the IL-23 signaling pathway, such as IL-23R, JAK2 and STAT3, have been characterized, but elements unique to this network as compared to other interleukins have not been readily explored. In this study, we have undertaken an integrative phosphoproteomics approach to better characterise downstream signaling events. To this end, we performed and compared phosphopeptide and phosphoprotein enrichment methodologies after activation of T lymphocytes by IL-23. We demonstrate the complementary nature of the two phosphoenrichment approaches by maximizing the capture of phosphorylation events. A total of 8202 unique phosphopeptides, and 4317 unique proteins were identified, amongst which STAT3, PKM2, CDK6 and LASP-1 showed induction of specific phosphorylation not readily observed after IL-2 stimulation. Interestingly, quantitative analysis revealed predominant phosphorylation of pre-existing STAT3 nuclear subsets in addition to translocation of phosphorylated STAT3 within 30 min after IL-23 stimulation. After IL-23R activation, a small subset of PKM2 also translocates to the nucleus and may contribute to STAT3 phosphorylation, suggesting multiple cellular responses including metabolic adaptation.
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Registered trials
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