ArticleInternational journal for parasitology2016
Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva.
Article in International journal for parasitology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Theileria parva genetics, prevalence and vaccination practices in Zimbabwe and the African region and the prospects for vaccine development: a systematic review.Veterinary research communications · 2025Pooled it
- Antigenic and genotypic relatedness of buffalo-derivedInternational journal for parasitology. Parasites and wildlife · 2026Article
- Article
- Systematic Determination of TCR-Antigen and Peptide-MHC Binding Kinetics among Field Variants of aJournal of immunology (Baltimore, Md. : 1950) · 2022Article
- Review
- Development of a Potential Yeast-Based Vaccine Platform forFrontiers in immunology · 2021Article
- Immune Response to Tick-Borne Hemoparasites: Host Adaptive Immune Response Mechanisms as Potential Targets for Therapies and Vaccines.International journal of molecular sciences · 2020Review
- Variant analysis of the sporozoite surface antigen gene reveals that asymptomatic cattle from wildlife-livestock interface areas in northern Tanzania harbour buffalo-derived T. parva.Parasitology research · 2020Article
- ExploringGenes · 2020Review
- Sequence diversity of cytotoxic T cell antigens and satellite marker analysis of Theileria parva informs the immunization against East Coast fever in Rwanda.Parasites & vectors · 2020Article
- Clinical Pathology, Immunopathology and Advanced Vaccine Technology in Bovine Theileriosis: A Review.Pathogens (Basel, Switzerland) · 2020Review
- Theileria parva: a parasite of African buffalo, which has adapted to infect and undergo transmission in cattle.International journal for parasitology · 2020Review
- Article
- Comparative Transcriptomics of the Bovine Apicomplexan ParasiteFrontiers in veterinary science · 2020Article
- Population Genetic Analysis of theFrontiers in microbiology · 2020Article
- Genetic and antigenic variation of the bovine tick-borne pathogen Theileria parva in the Great Lakes region of Central Africa.Parasites & vectors · 2019Article
- Infection and treatment method (ITM) vaccine against East Coast fever: reducing the number of doses per straw for use in smallholder dairy herds by thawing, diluting and refreezing already packaged vaccine.BMC veterinary research · 2019Article
- Molecular and Antigenic Properties of Mammalian Cell-ExpressedFrontiers in immunology · 2019Article
- Genetic Profiling Reveals High Allelic Diversity, Heterozygosity and Antigenic Diversity in the Clinical Isolates of theFrontiers in physiology · 2019Article
- Theileria parva antigens recognized by CD8+ T cells show varying degrees of diversity in buffalo-derived infected cell lines.Parasitology · 2018Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
An infection and treatment protocol is used to vaccinate cattle against Theileria parva infection. Due to incomplete cross-protection between different parasite isolates, a mixture of three isolates, termed the Muguga cocktail, is used for vaccination. While vaccination of cattle in some regions provides high levels of protection, some animals are not protected against challenge with buffalo-derived T. parva. Knowledge of the genetic composition of the Muguga cocktail vaccine is required to understand how vaccination is able to protect against field challenge and to identify the potential limitations of the vaccine. The aim of the current study was to determine the extent of genetic and antigenic diversity within the parasite isolates that constitute the Muguga cocktail. High throughput multi-locus sequencing of antigen-encoding loci was performed in parallel with typing using a panel of micro- and mini-satellite loci. The former focused on genes encoding CD8(+) T cell antigens, believed to be relevant to protective immunity. The results demonstrate that each of the three component stocks of the cocktail contains limited parasite genotypic diversity, with single alleles detected at many gene/satellite loci and, moreover, that two of the components show a very high level of similarity. Thus, the vaccine incorporates very little of the genetic and antigenic diversity observed in field populations of T. parva. The presence of alleles at low frequency (<10%) within vaccine component populations also points to the possibility of variability in the content of vaccine doses and the potential for loss of allelic diversity during tick passage. The results demonstrate that there is scope to modify the content of the vaccine in order to enhance its diversity and thus its potential for providing broad protection. The ability to accurately quantify genetic diversity in vaccine component stocks will facilitate improved quality control procedures designed to ensure the long-term efficacy of the vaccine.
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