ArticleInternational journal of legal medicine2016
PCR-based identification of drowning: four case reports.
Article in International journal of legal medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 32 citations in OpenAlex.
- Forensic species identification: practical guide for animal and plant DNA analysis.International journal of legal medicine · 2024Article
- Trends in forensic microbiology: From classical methods to deep learning.Frontiers in microbiology · 2023Review
- Use of cadaveric vitreous humor as an innovative substrate for diatoms research and forensic diagnosis of drowning.International journal of legal medicine · 2022Article
- Research advances in forensic diatom testing.Forensic sciences research · 2020Review
- Microbial forensics: new breakthroughs and future prospects.Applied microbiology and biotechnology · 2018Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proper diagnosis in drowning victims is often difficult due to the lack of signs specific to drowning. The diatom test is a widely used procedure for the diagnosis. Some types of water contain only minimal amounts of diatom cells which may provide false-negative results, while a negative diatom test result does not exclude drowning. In proving drowning, we used a polymerase chain reaction (PCR)-based biological method in addition to the conventional methods. DNA was extracted from postmortem spleen tissues and water of the drowning site. Samples were tested with algae (diatoms and small green algae)- and cyanobacteria (blue-green algae)-specific primers. We present here multiple drowning cases in which diatom tests of the postmortem tissue samples and the water were negative. In each case, the presence of phytoplanktonic DNA strengthened the autopsy diagnosis of drowning even in the absence of visible diatoms. In the future, the PCR method may be of consideration as a possible supplement of the diatom test in the examination of presumed drowning cases.
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Registered trials
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