ArticleBiochimica et biophysica acta2016
Tumor necrosis factor reduces Plasmodium falciparum growth and activates calcium signaling in human malaria parasites.
Article in Biochimica et biophysica acta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
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- Cellular Immune Response and T Cell Epitope Mapping ofVaccines · 2026Article
- Reprogramming of host energy metabolism mediated by the TNF-iNOS-HIF-1α axis plays a key role in host resistance tobioRxiv : the preprint server for biology · 2026Article
- Hidden hematological, biochemical and immune costs of asymptomatic malaria infections in semi-wild chimpanzees.bioRxiv : the preprint server for biology · 2025Article
- Comparative immunological study of Plasmodium knowlesi infections in humans and macaques: insights into cytokine dynamics.Malaria journal · 2025Article
- Cytokine gene polymorphisms implicated in the pathogenesis ofFrontiers in immunology · 2024Review
- Profiles of host immune impairment inHeliyon · 2022Review
- Identification of a TNF-TNFR-like system in malaria vectors (Anopheles stephensi) likely to influence Plasmodium resistance.Scientific reports · 2022Article
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- The Role of Purinergic P2X7 Receptor in Inflammation and Cancer: Novel Molecular Insights and Clinical Applications.Cancers · 2022Review
- Malaria parasite infection compromises colonization resistance to an enteric pathogen by reducing gastric acidity.Science advances · 2021Article
- P2RX7 at the Host-Pathogen Interface of Infectious Diseases.Microbiology and molecular biology reviews : MMBR · 2021Review
- Recent Advances in Understanding the Inflammatory Response in Malaria: A Review of the Dual Role of Cytokines.Journal of immunology research · 2021Review
- Zoonotic and vector-borne parasites and epilepsy in low-income and middle-income countries.Nature reviews. Neurology · 2020Review
- Testing possible causes of gametocyte reduction in temporally out-of-synch malaria infections.Malaria journal · 2020Article
- Asymptomatic Malaria Infection Is Maintained by a Balanced Pro- and Anti-inflammatory Response.Frontiers in microbiology · 2020Article
- TNF-α promoter polymorphisms (G-238A and G-308A) are associated with susceptibility to Systemic Lupus Erythematosus (SLE) and P. falciparum malaria: a study in malaria endemic area.Scientific reports · 2019Article
- Antierythropoietin Antibody Production Is Not Associated with Malaria and Malaria-Related Anaemia in Humans.TheScientificWorldJournal · 2019Article
- Comparative 1D Blue-Native electrophoresis analysis of Plasmodium falciparum and human proteins associated with cytoadherence.Malaria journal · 2018Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlasmodium has a complex biology including the ability to interact with host signals modulating their function through cellular machinery. Tumor necrosis factor (TNF) elicits diverse cellular responses including effects in malarial pathology and increased infected erythrocyte cytoadherence. As TNF levels are raised during Plasmodium falciparum infection we have investigated whether it has an effect on the parasite asexual stage.
methodsFlow cytometry, spectrofluorimetric determinations, confocal microscopy and PCR real time quantifications were employed for characterizing TNF induced effects and membrane integrity verified by wheat germ agglutinin staining.
resultsTNF is able to decrease intracellular parasitemia, involving calcium as a second messenger of the pathway. Parasites incubated for 48 h with TNF showed reduced erythrocyte invasion. Thus, TNF induced rises in intracellular calcium concentration, which were blocked by prior addition of the purinergic receptor agonists KN62 and A438079, or interfering with intra- or extracellular calcium release by thapsigargin or EGTA (ethylene glycol tetraacetic acid). Importantly, expression of PfPCNA1 which encodes the Plasmodium falciparum Proliferating-Cell Nuclear Antigen 1, decreased after P. falciparum treatment of TNF (tumor necrosis factor) or 6-Bnz cAMP (N(6)-benzoyladenosine-3',5'-cyclic monophosphate sodium salt).
conclusionsThis is potentially interesting data showing the relevance of calcium in downregulating a gene involved in cellular proliferation, triggered by TNF. GENERAL SIGNIFICANCE: The data show that Plasmodium may subvert the immunological system and use TNF for the control of its proliferation within the vertebrate host.
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