Evidence map›Paper›PMID 27080007›Full record

ArticleJournal of the American Society for Mass Spectrometry2016

Revealing Higher Order Protein Structure Using Mass Spectrometry.

Brian T Chait, Martine Cadene, Paul Dominic Olinares, Michael P Rout, Yi Shi

Abstract read
In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed.

  1. Polyproline peptide targetsCell reports. Physical science · 2024
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  8. Defining principles that influence antimicrobial peptide activity against capsulatedProceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  9. Article
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  11. Review
  12. Review
  13. Article
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  16. Mapping low-affinity/high-specificity peptide-protein interactions using ligand-footprinting mass spectrometry.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  17. Review
  18. Article
  19. MetaUniDec: High-Throughput Deconvolution of Native Mass Spectra.Journal of the American Society for Mass Spectrometry · 2019
    Article
  20. Liquid Native MALDI Mass Spectrometry for the Detection of Protein-Protein Complexes.Journal of the American Society for Mass Spectrometry · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Brian T ChaitLaboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY, 10065, USA. chait@rockefeller.edu.ORCID 0000-0003-3524-557X
Martine CadeneCBM, CNRS UPR4301, Rue Charles Sadron, CS 80054, 45071, Orleans Cedex 2, France.
Paul Dominic OlinaresLaboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY, 10065, USA.
Michael P RoutLaboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY, 10065, USA.
Yi ShiLaboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY, 10065, USA.

Funding

TR&D Project 4. The Imaging Stage: Multiscale Spatiotemporal Modeling of Macromolecular Systems in Cellular NeighborhoodsP41GM109824 · NIGMS · ROCKEFELLER UNIVERSITY · PI ROUT, MICHAEL P · 2014 to 2023
$18.8M
YNG1 PHD BINDING TO H3 3METHYL AT K4 PROMOTES TRANSCRIPTION AT TARGETED ORFSP41RR000862 · NCRR · ROCKEFELLER UNIVERSITY · PI FENYO, DAVID · 1985 to 2011
$16.1M
National Resource for Mass Spectrom of Biological MacromoleculesP41GM103314 · NIGMS · ROCKEFELLER UNIVERSITY · PI CHAIT, BRIAN T · 2012 to 2017
$9.9M
NCRR NIH HHS P41 RR000862NIGMS NIH HHS P41 GM103314NIGMS NIH HHS P41 GM109824
6 · The paper itself

Abstract

The development of rapid, sensitive, and accurate mass spectrometric methods for measuring peptides, proteins, and even intact protein assemblies has made mass spectrometry (MS) an extraordinarily enabling tool for structural biology. Here, we provide a personal perspective of the increasingly useful role that mass spectrometric techniques are exerting during the elucidation of higher order protein structures. Areas covered in this brief perspective include MS as an enabling tool for the high resolution structural biologist, for compositional analysis of endogenous protein complexes, for stoichiometry determination, as well as for integrated approaches for the structural elucidation of protein complexes. We conclude with a vision for the future role of MS-based techniques in the development of a multi-scale molecular microscope. Graphical Abstract ᅟ.

Indexed as

Mass SpectrometryPeptidesProteinsSpectrometry, Mass, Electrospray IonizationPeptidesProteinsElectrospray ionizationEndogenous protein complexesFolding state in solutionHigher order structureMALDIMass spectrometryMolecular microscopeNative mass spectrometryProtein assemblyProtein complexProtein compositionStoichiometryStructural biologyX-ray crystallography

Identifiers

PMID27080007
PMCPMC5125627

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.