ArticleNature communications2016
Characterization of eukaryotic DNA N(6)-methyladenine by a highly sensitive restriction enzyme-assisted sequencing.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 115 citations in OpenAlex.
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- Genome-wide chromatin recording resolves dynamic cell state changes.bioRxiv : the preprint server for biology · 2026Article
- A pentose, as a cytosine nucleobase modification inApplied and environmental microbiology · 2026Article
- Epigenome analysis of an algae-infecting giant virus reveals a unique methylation motif catalogue.PloS one · 2025Article
- Sequencing of NMolecular cell · 2024Article
- 6mA-Sniper: Quantifying 6mA sites in eukaryotes at single-nucleotide resolution.Science advances · 2023Article
- Single-Nucleotide Resolution Mapping ofACS central science · 2023Article
- Epigenetic Regulation and Chromatin Remodeling in Malaria Parasites.Annual review of microbiology · 2023Review
- Force-Induced Visualization of Nucleic Acid Functions with Single-Nucleotide Resolution.Sensors (Basel, Switzerland) · 2023Article
- The Global Changes of N6-methyldeoxyadenosine in Response to Low Temperature inPlants (Basel, Switzerland) · 2023Article
- Navigating the pitfalls of mapping DNA and RNA modifications.Nature reviews. Genetics · 2023Review
- Mammalian DNA NMolecular cell · 2023Review
- High-precision mapping reveals rare NCell discovery · 2022Article
- Light Dependent Changes in Adenylate Methylation of the Promoter of the Mitochondrial Citrate Synthase Gene in Maize (International journal of molecular sciences · 2022Article
- Review
- NT-seq: a chemical-based sequencing method for genomic methylome profiling.Genome biology · 2022Article
- Critical assessment of DNA adenine methylation in eukaryotes using quantitative deconvolution.Science (New York, N.Y.) · 2022Article
- Femtomolar and locus-specific detection of NJournal of nanobiotechnology · 2021Article
- Using k-mer embeddings learned from a Skip-gram based neural network for building a cross-species DNA N6-methyladenine site prediction model.Plant molecular biology · 2021Article
- The exploration of N6-deoxyadenosine methylation in mammalian genomes.Protein & cell · 2021Review
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Although extensively studied in prokaryotes, the prevalence and significance of DNA N(6)-methyladenine (6mA or m(6)dA) in eukaryotes had been underappreciated until recent studies, which have demonstrated that 6mA regulates gene expression as a potential heritable mark. To interrogate 6mA sites at single-base resolution, we report DA-6mA-seq (DpnI-Assisted N(6)-methylAdenine sequencing), an approach that uses DpnI to cleave methylated adenine sites in duplex DNA. We find that DpnI cuts other sequence motifs besides the canonical GATC restriction sites, thereby expanding the utility of this method. DA-6mA-seq achieves higher sensitivity with nanograms of input DNA and lower sequencing depth than conventional approaches. We study 6mA at base resolution in the Chlamydomonas genome and apply the new method to two other eukaryotic organisms, Plasmodium and Penicillium. Combined with conventional approaches, our method further shows that most 6mA sites are fully methylated on both strands of DNA at various sequence contexts.
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